A kind of pumpkin free microspore induction medium and induction culture method

A technology of induction medium and culture method, which is applied in the field of pumpkin free microspore induction medium and induction culture, and can solve the problems of low microspore embryogenic callus induction rate, instability, slow progress in the research of pumpkin free microspore culture, etc. , to achieve high industrial promotion value, improve induction efficiency, and increase induction rate

Active Publication Date: 2020-11-20
泗县微腾知识产权运营有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the progress of pumpkin free microspore culture research is slow, and the research on pumpkin breeding using this technology is rarely reported. The main reason is that the microspore embryogenic callus induction rate is low and unstable, which directly limits the pumpkin free microspore culture technology in breeding. application on

Method used

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  • A kind of pumpkin free microspore induction medium and induction culture method
  • A kind of pumpkin free microspore induction medium and induction culture method
  • A kind of pumpkin free microspore induction medium and induction culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Prepare a kind of pumpkin free microspore induction medium of the present invention, and the medium formula is specific as follows:

[0025] KNO 3 85mg / L, MgSO 4 ∙7H 2 O 150mg / L, KH 2 PO 4 73mg / L, Ca(NO 3 ) 2 ∙4H 2 O 180mg / L, MnSO 4 ∙4H 2 O 27.5mg / L, Na 2 -EDTA 12.5mg / L, FeSO 4 ∙7H 2 O 13.5mg / L, H 3 BO 3 8.25mg / L, ZnSO 4 ∙7H 2 O9.0mg / L, KI0.5mg / L, AgNO 3 17mg / L, Na 2 MoO 4 ∙2H 2 O 0.25mg / L, CuSO 4 ∙5H 2 O 0.025mg / L, CoCl 2 ∙6H 2 O 0.025mg / L, Adenine 15mg / L, L-Glutamine 500mg / L, Vitamin B1 1.0mg / L, Vitamin B6 0.7mg / L, Vc 0.6mg / L, Niacin 5.0mg / L, Inositol 170mg / L, glycine 4.0mg / L, cyclic guanylic acid 0.2g / L, glutathione 32.5mg / L, methylnitrosourea 1.5g / L, sucrose 60g / L, plant gel 3.25g / L L, 6-BA 0.2mg / L, NAA 0.35mg / L, mannitol 12g / L, polyethylene glycol 160mg / L, hydrolyzed protein 1.2g / L, phytosulfokinetin PSK-α 30pmol / L, pH 5.6 .

[0026] The induction culture method of pumpkin free microspores is:

[0027] Sow the pumpkin seeds in the fie...

Embodiment 2

[0041] In order to compare the difference between pumpkin free microspore induction medium provided by the present invention and other mediums to the induction efficiency of pumpkin free microspore embryogenic callus, we have selected 2 kinds of free microspore induction medium in addition, and the test varieties also adopt For Jinhong No. 1 and Jinjin No. 2, the isolation method, tissue culture operation method and culture method of free microspores are all the same as in Example 1. After the embryogenic callus is formed, the microspores of Jinhong No. 1 and Jinjin No. 2 are counted respectively Microspore callus induction rate in the two media.

[0042] The other 2 free microspore induction medium formulations are:

[0043] NLN culture medium, pH is 5.8 (the comparison document comes from "Chinese Cabbage Free Microspore Culture" by Li Genyi et al.);

[0044] NLN-13 medium + 0.5g / L activated carbon, the pH is 5.8 (the comparison document comes from Jiang Wusheng et al. "The...

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Abstract

The invention provides an isolated microspore induction medium and isolated microspore induction culture method for Cucurbita moschata; the medium comprises KNO3, MgSO4 7H2O, KH2PO4, Ca(NO3)2 4H2O, MnSO4 4H2O, Na2 EDTA, FeSO4 7H2O, H3BO3, ZnSO4 7H2O, KI, AgNO3, Na2MoO4 2H2O, CuSO4 5H2O, CoCl2 6H2O, adenine, L-glutamine, vitamin B1, vitamin B6, Vc, nicotinic acid, inositol, glycine, cyclic guanosine monophosphate, glutathione, N-nitroso-N-methylurea, sucrose, phytagel, 6-BA, NAA, mannitol, polyethylene glycol, protein hydrolysate, and phytosulfokine-alpha (PSK-alpha). Embryonic callus induction rate of isolated microspores of Cucurbita moschata is improved significantly, and the establishment of an anther culture breeding system of Cucurbita moschata is promoted.

Description

technical field [0001] The invention relates to a pumpkin free microspore induction medium and an induction culture method, in particular to a pumpkin free microspore induction culture which significantly improves the induction efficiency of pumpkin free microspore embryogenic callus and promotes the establishment of pumpkin flower breeding system The invention relates to a method for cultivating substrates and induction, and belongs to the high-tech field of vegetable cultivation. Background technique [0002] pumpkin( Cucurbita moschata ) is an annual vine herb belonging to Cucurbitaceae Cucurbitaceae, which originated in America and was introduced to China in the mid-16th century. There are currently five types of pumpkins cultivated in the world: Chinese pumpkins, Indian pumpkins, American pumpkins, gray-seeded pumpkins and black-seeded pumpkins. In China, the first three are mainly cultivated. Pumpkin is one of the earliest ancient crops cultivated by humans. The cult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
CPCC12N5/0025C12N5/04
Inventor 刘汉平张超李峰
Owner 泗县微腾知识产权运营有限公司
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