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Chinese cabbage isolated microspore inducing medium formula

A technology for inducing culture medium and microspores, which is applied in the field of Chinese cabbage free microspore induction medium formulation field, and can solve the problem of low embryoid seedling rate, low microspore induction rate, imperfect Chinese cabbage free microspore culture technology, etc. problem, to achieve the effect of improving the induction rate and the induction ability

Inactive Publication Date: 2017-02-22
安徽轩之界农业发展有限公司
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AI Technical Summary

Problems solved by technology

Although Chinese cabbage dissociated microspore culture and breeding technology has made a series of research progress, and has also bred excellent varieties such as Yubaicai No. , mainly because the induction rate of free microspores of Chinese cabbage is not high, and the problems such as the low rate of embryoid seedling formation have not been well resolved.
The low microspore induction rate will make it difficult to obtain enough selection populations, which directly limits the application of Chinese cabbage free microspore culture technology in breeding

Method used

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  • Chinese cabbage isolated microspore inducing medium formula

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Embodiment

[0019] Prepare a kind of Chinese cabbage free microspore induction medium of the present invention, and the medium formula is specific as follows:

[0020] KNO 3 60mg / L, MgSO 4 ∙7H 2 O 62.5mg / L, KH 2 PO 4 67.5mg / L, Ca(NO 3 ) 2 ∙4H 2 O 225mg / L, Na 2 -EDTA 12.5mg / L, FeSO 4 ∙7H 2 O 13.5mg / L, MnSO 4 ∙4H 2 O 25mg / L, AgNO 3 8mg / L,H 3 BO 3 8.25mg / L, ZnSO 4 ∙7H 2 O 9.0mg / L, KI 0.425mg / L, Na 2 MoO 4 ∙2H 2 O 0.25mg / L, CuSO 4 ∙5H 2 O 0.025mg / L, CoCl 2 ∙6H 2 O 0.025mg / L, L-glutamine 800mg / L, vitamin B1 0.5mg / L, vitamin B6 0.5mg / L, niacin 5mg / L, folic acid 0.05mg / L, biotin 0.05mg / L, inositol 100mg / L, glycine 4mg / L, proline 20mg / L, serine 80mg / L, L-glutathione 30mg / L, methylnitrosourea 1.5g / L, sucrose 130g / L, vegetable gel 3.25g / L, 6-BA 0.25mg / L, complex nucleic acid 0.2mg / L, hydrolyzed protein 0.6g / L, colchicine 0.07mg / L, activated carbon 0.05g / L, plant sulfokinetin PSK-α 35pmol / L, pH 5.7.

[0021] In April 2015, at the initial flowering stage of Chinese cabb...

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Abstract

The invention provides a Chinese cabbage isolated microspore inducing medium. According to the formula, the medium contains KNO3, MgSO4-7H2O, KH2PO4, Ca(NO3)2-4H2O, Na2-EDTA, FeSO4-7H2O, MnSO4-4H2O, AgNO3, H3BO3, ZnSO4-7H2O, KI, Na2MoO4-2H2O, CuSO4-5H2O, CoCl2-6H2O, L-glutamine, vitaminB1, vitaminB6, nicotinic acid, folic acid, biotin, inositol, glycine, proline, serine, L-glutathione, methylnitrosourea, sucrose, phytagel, 6-BA, complex peptide nucleic acid, protein hydrolysate, colchicines, activated carbon and plant vulcanized kinetin PSK-alpha. The medium can greatly improve the inducing efficiency of Chinese cabbage isolated microspore embryonic calluses and has important application value.

Description

technical field [0001] The invention relates to a Chinese cabbage free microspore induction medium formula, in particular to a medium formula that can greatly improve the efficiency of Chinese cabbage free microspore induction into embryogenic callus, and belongs to the high-tech field of vegetable cultivation. Background technique [0002] Chinese cabbage (Brassica campestris ssp. pekinensis Lour.) belongs to the Brassicaceae Brassica species Brassica subspecies, and is an important vegetable crop native to my country. Chinese cabbage has various ecological types, wide distribution, high yield, storage and transportation resistance, long supply period, comprehensive nutrition, various eating methods, easy planting, labor saving, and low cost. It occupies an irreplaceable important position in my country's vegetable basket. The cultivation area and output of Chinese cabbage are the first among all kinds of vegetable crops in my country, and the production of Chinese cabbage ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
CPCC12N5/0025C12N2500/05C12N2500/30C12N2500/32C12N2500/35C12N2500/38C12N2500/40
Inventor 宋兆霞谭兴江张瑞明
Owner 安徽轩之界农业发展有限公司
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