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Formula of free microspore induced culture medium for brassica campestris

A technology for inducing culture medium and microspores, which is applied in the field of free microspore induction medium formulation of pakchoi, can solve the problems of slow research progress, low induction rate and limitation of microspore embryogenic callus, and achieves improved induction efficiency, higher The effect of industrial promotion value

Inactive Publication Date: 2017-01-04
张瑞明
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Problems solved by technology

However, in recent years, the research on the cultivation of free microspores of pakchoi has been slow, and there are few reports on the use of this technology for the breeding of pakchoi. Application of Spore Culture Technology in Breeding

Method used

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  • Formula of free microspore induced culture medium for brassica campestris

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Embodiment

[0019] Prepare a kind of pakchoi free microspore induction medium of the present invention, and the medium formula is specific as follows:

[0020] KNO 3 60mg / L, MgSO 4 ∙7H 2 O 62.5mg / L, KH 2 PO 4 67.5mg / L, Ca(NO 3 ) 2 ∙4H 2 O 225mg / L, Na 2 -EDTA 12.5mg / L, FeSO 4 ∙7H 2 O 13.5mg / L, MnSO 4 ∙4H 2 O 25mg / L, AgNO 3 17mg / L,H 3 BO 3 8.25mg / L, ZnSO 4 ∙7H 2 O 9mg / L, KI 0.425mg / L, Na 2 MoO 4 ∙2H 2 O 0.25mg / L, CuSO 4 ∙5H 2 O 0.025mg / L, CoCl 2 ∙6H 2 O 0.025mg / L, 2-hydroxyethyltrimethylammonium chloride 140mg / L, L-glutamine 500mg / L, vitamin B1 0.5mg / L, vitamin B6 0.5mg / L, niacin 5mg / L, folic acid 0.05 mg / L, biotin 0.05mg / L, inositol 160mg / L, glycine 4mg / L, L-glutathione 30mg / L, methylnitrosourea 1.5g / L, sucrose 130g / L, plant gel Glue 3.25g / L, 6-BA 0.2mg / L, NAA 0.35mg / L, polyethylene glycol 0.16g / L, hydrolyzed protein 1.5g / L, activated carbon 0.02g / L, plant sulfokinetin PSK-α 45pmol / L, pH 5.4.

[0021] In the autumn of 2013, the seeds of the pakchoi varieties ...

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Abstract

The invention provides a free microspore induced culture medium for brassica campestris. A formula of the culture medium contains KNO3, MgSO4.7H2O, KH2PO4, Ca(NO3)2.4H2O, Na2-EDTA, FeSO4.7H2O, MnSO4.4H2O, AgNO3, H3BO3, ZnSO4.7H2O, KI, Na2MoO4.2H2O, CuSO4 .5H2O, CoCl2.6H2O, chlorinated 2-hydroxyethyl trimethylamine, L-glutamine, vitamin B1, vitamin B6, nicotinic acid, folic acid, biotin, inositol, glycine, L-glutathione, methyl nitrosourea, sucrose, phytagel, 6-BA, NAA, polyethylene glycol, protein hydrolysate, active carbon and phytosulfokine PSK-alpha. The culture medium can be used for greatly improving the induction efficiency of free microspore embryogenic calli of brassica campestris, and has an important application value.

Description

technical field [0001] The invention relates to a pakchoi free microspore induction medium formula, in particular to a pakchoi free microspore induction medium formula that can greatly improve the induction efficiency of pakchoi free microspore embryogenic callus and has important application value. It belongs to the high-tech field of vegetable cultivation. Background technique [0002] Chinese cabbage ( Brassica campestris L. ssp. chinensis ) belongs to Brassica Brassica Brassica subspecies of Brassica cabbage, also known as non-heading cabbage, green cabbage, small rapeseed, including Chinese cabbage (common cabbage), Chinese cabbage, Chinese cabbage, Chinese cabbage and other varieties, native to my country is a vegetable crop widely cultivated in various parts of our country. Because of its strong edibility, rich nutrition, high quality, and various flavors, it is deeply loved by people. Moreover, pakchoi has a short production period, strong adaptability, and low p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张瑞明宋兆霞谭兴江
Owner 张瑞明
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