Corn difference peel color CMS three line breeding and application thereof in mixed-planting production
A peel and difference technology, applied in the field of corn hybrid seed production, can solve the problems of complex production mode operation, low degree of mechanization, high cost, etc., and achieve the effect of being suitable for mechanized operation, simplifying production mode and ensuring quality
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Embodiment 1
[0051] 1. Breeding and production utilization of brown (P-bpbp) pericarp CMS male sterile line GN501 Brown A
[0052] 1) The white (pp--) pericarp CMS maintainer line GN01B with excellent comprehensive agronomic traits was used as the recurrent parent, and the maize inbred line PB502 with brown (P-bpbp) pericarp was crossed, backcrossed for 4 generations and then selfed for 2 generations. The brown (P-bpbp) pericarp maintainer line GN01 Brown B was bred;
[0053] 2) Using GN01 brown skin B as the recurrent male parent, it was paired with the isotype male sterile line GN01A of the original white (pp--) peel maintainer line GN01B, and the breeding sterility traits were stable and thorough and brown (P-bpbp) CMS male sterile line GN01 Brown A with peel traits.
[0054] 3) Mix the seeds of the white (pp--) pericarp CMS restorer line GN01R and the sterile line GN01 brown skin A according to the ratio of parent parent 1: (4-6), and then use precision seeding machinery to mix and so...
Embodiment 2
[0056] 2. Breeding and production utilization of red (P-bpbp) pericarp CMS restorer line GN501 Hongpi R
[0057] 1) The white (pp--) peel color CMS restorer line GN501R with excellent comprehensive agronomic traits was used as the recurrent parent, crossed with the maize inbred line 81162 with red (P-Bp-) peel, backcrossed for 4 generations and then selfed for 4 Generation, the red (P-Bp-) peel restorer line GN01 red skin R was bred;
[0058] 2) Mix the seeds of the red (P-Bp-) pericarp restorer line GN01 red-skin R and the white (pp--) pericarp male sterile line GN01A according to the ratio of parent parent 1: (4-6), and use The precision seeding machinery is mixed in the seed production field, and the field management is the same as the field production; the male sterile line seeds are harvested mechanically; in the process of seed processing, according to the difference in the color of the peel, combined with ear selection, manual selection and removal of red seeds (red pee...
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