Method for collecting intestinal flora of inhuman primates in free state

Abstract

The invention provides a method for collecting the intestinal flora of inhuman primates in a free state. The method comprises the following steps: (1) placing a flexible pad towel and a hard backing board for bearing the flexible pad towel at the bottom of a feeding container of an inhuman primate, wherein the flexible pad towel comprises a water absorbing layer and a waterproof layer, and the waterproof layer is in contact with the hard backing board; and (2) after the defecation of the animal is observed, taking the hard backing board and the flexible pad towel out of the feeding container, and collecting the intestinal flora sample on the flexible pad towel. When the feces and urine of the animal under a free active state fall onto the flexible pad towel, the urine is absorbed by the water absorbing layer to prevent the feces from being mixed with the urine into paste; the waterproof layer can keep the original dryness of the caprophyl and is favorable for keeping the integrity of the intestinal flora sample; and the hard backing board is convenient for taking out the flexible pad towel and collecting the intestinal flora sample on the flexible pad towel. The method has the advantages of big sampling amount, good repeated sampling performance and good integrity of a flora gene sample.

Description

Technical field [0001] The present invention relates to the technical field of collection of animal intestinal flora, in particular to a method for collecting intestinal flora of non-human primates in a free state. Background technique [0002] The human intestine is a diversified and vibrant micro-ecological system, with many kinds of bacterial intestinal flora. Intestinal flora has an important impact on human health. It has been reported to be closely related to cardiovascular and cerebrovascular diseases, obesity, diabetes, AIDS, autism, depression and Alzheimer’s. Therefore, the study of intestinal flora has become Research hotspots in recent years. [0003] Non-human primates (mainly monkeys) are the closest to humans due to their genetic relationship, 75%-85% homology with human genetic material, and highly similar to humans in terms of physiological structure and immune metabolism , Compared with other experimental animals, it has a powerful advantage in brain cognition r...

AI Technical Summary

Problems solved by technology

Method 1) usually picks up the feces at the bottom of the monkey cage at a fixed time (every morning), this method can be repeated for a long time, and the sampling volume is high; but the disadvantage is that the feces dropped at the bottom of the monkey cage are usually mixed with urine Together, it turns into a paste whose moist texture and prolonged storage are not conducive to maintaining the integrity of gut flora genetic samples

Method 2) Usually, a cotton swab is used to go deep into the anus and reach the rectum to collect fecal bacteria. This method can quickly freeze the intestinal flora samples in a short time to ensure the integrity of the flora gene samples, but the sampling volume of the cotton swab is small and the sampling efficiency is low

Method 3) can guarantee both the integrity of the flora gene sample and the sampling volume, but since the animal has been sacrificed, repeated sampling cannot be repeated for a long time, and the long-term detection of the intestinal flora of the animal becomes impossible

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