Stem cell extract-containing dressing material for wound repair and preparation method thereof

Abstract

The invention belongs to the field of medical dressings, and particularly relates to a stem cell extract-containing dressing material for wound repair and a preparation method thereof. The stem cell extract-containing dressing material for wound repair (per 100g) is prepared from the following components: 1 to 10g of stem cell extract, 1 to 5 g of chitosan quaternary ammonium salt, 5 to 10g of bioactive glass powder, 0.3 to 0.5g of jojoba oil, 5.5 to 6.5g of glycerin and 65 to 82.2g of distilled water. The dressing provided by the invention can effectively promote wound healing and has obvious sterilization and disinfection functions. In addition, the dressing disclosed by the invention is a liquid dressing, which is convenient to use on various wounds in the skin.

Description

technical field

[0001] The invention belongs to the field of medical dressings, in particular to a dressing containing stem cell extracts for wound repair and a preparation method thereof. Background technique

[0002] The skin is the largest organ of the human body. The skin covers the whole body and is in contact with the external environment. It acts as a barrier to protect the body. It protects various tissues and organs in the body from physical, mechanical, chemical and pathogenic microorganisms. , such as preventing the loss of water, electrolytes and plasma proteins, resisting the invasion of bacteria, preventing the entry of poisons, resisting mechanical damage, and preventing the damage of ultraviolet radiation to the human body. In addition, the skin also has physiological functions such as sensation, secretion, excretion and temperature regulation. Therefore, whether the skin is an ulcer wound or various burns, abrasions, etc., it should be taken care of as soon...

Examples

Embodiment 1

[0028] Example 1 A dressing containing stem cell extracts for wound repair

[0029] The dressing containing stem cell extracts for wound repair, each 100g of the dressing is composed of the following components and their weight: 8g of induced pluripotent stem cell extracts, 3g of chitosan quaternary ammonium salts, 10g of bioactive glass powder, and Jojoba oil 0.3g, glycerin 6.2g and distilled water 68g.

[0030] The preparation method of described IPS cell extract is:

[0031] S1. Cultivation of induced pluripotent stem cells: after Matrigel was dissolved overnight at 4°C, dilute Matrigel with DMEM / F12 at a volume ratio of 1:100, mix well, add to a culture dish to coat for 30 minutes, and discard the culture medium , inoculated induced pluripotent stem cells, and incubated at 37°C in CO 2 Cultivated in an incubator;

[0032] S2. Cell extract extraction: suck out the culture medium in the S3 culture bottle, wash it with PBS for 2-3 times, and homogenize it for 3-5 times at be...

Embodiment 2

[0042] Example 2 A dressing containing stem cell extracts for wound repair

[0043] The dressing containing stem cell extracts for wound repair, each 100g of the dressing is composed of the following components and their weight: 1g of induced pluripotent stem cell extract, 5g of chitosan quaternary ammonium salt, 5g of bioactive glass powder, and Jojoba oil 0.3g, glycerin 5.5g and distilled water 82.2g.

[0044] The preparation method of described IPS cell extract is:

[0045] S1. Cultivation of induced pluripotent stem cells: after Matrigel was dissolved overnight at 4°C, dilute Matrigel with DMEM / F12 at a volume ratio of 1:100, mix well, add to a culture dish to coat for 30 minutes, and discard the culture medium , inoculated induced pluripotent stem cells, and incubated at 37°C in CO 2 Cultivated in an incubator;

[0046] S2. Cell extract extraction: suck out the culture medium in the S3 culture bottle, wash it with PBS for 2-3 times, and homogenize it for 3-5 times at b...

Embodiment 3

[0050] Example 3 A dressing containing stem cell extracts for wound repair

[0051] The dressing containing stem cell extracts for wound repair, each 100g of the dressing is composed of the following components and their weight: 10g of induced pluripotent stem cell extract, 1g of chitosan quaternary ammonium salt, 10g of bioactive glass powder, and Jojoba oil 0.5g, glycerin 6.5g and distilled water 65g.

[0052] The preparation method of described IPS cell extract is:

[0053] S2. Cultivation of induced pluripotent stem cells: after Matrigel was dissolved overnight at 4°C, dilute Matrigel with DMEM / F12 at a volume ratio of 1:100, mix well, add to a culture dish to coat for 30 minutes, and discard the culture medium , inoculated induced pluripotent stem cells, and incubated at 37°C in CO 2 Cultivated in an incubator;

[0054] S2. Cell extract extraction: suck out the culture medium in the S3 culture bottle, wash it with PBS for 2-3 times, and homogenize it for 3-5 times at b...

technical field

[0001] The invention belongs to the field of medical dressings, in particular to a dressing containing stem cell extracts for wound repair and a preparation method thereof. Background technique

[0002] The skin is the largest organ of the human body. The skin covers the whole body and is in contact with the external environment. It acts as a barrier to protect the body. It protects various tissues and organs in the body from physical, mechanical, chemical and pathogenic microorganisms. , such as preventing the loss of water, electrolytes and plasma proteins, resisting the invasion of bacteria, preventing the entry of poisons, resisting mechanical damage, and preventing the damage of ultraviolet radiation to the human body. In addition, the skin also has physiological functions such as sensation, secretion, excretion and temperature regulation. Therefore, whether the skin is an ulcer wound or various burns, abrasions, etc., it should be taken care of as soon...

Examples

Embodiment 1

[0028] Example 1 A dressing containing stem cell extracts for wound repair

[0029] The dressing containing stem cell extracts for wound repair, each 100g of the dressing is composed of the following components and their weight: 8g of induced pluripotent stem cell extracts, 3g of chitosan quaternary ammonium salts, 10g of bioactive glass powder, and Jojoba oil 0.3g, glycerin 6.2g and distilled water 68g.

[0030] The preparation method of described IPS cell extract is:

[0031] S1. Cultivation of induced pluripotent stem cells: after Matrigel was dissolved overnight at 4°C, dilute Matrigel with DMEM / F12 at a volume ratio of 1:100, mix well, add to a culture dish to coat for 30 minutes, and discard the culture medium , inoculated induced pluripotent stem cells, and incubated at 37°C in CO 2 Cultivated in an incubator;

[0032] S2. Cell extract extraction: suck out the culture medium in the S3 culture bottle, wash it with PBS for 2-3 times, and homogenize it for 3-5 times at be...

Embodiment 2

[0042] Example 2 A dressing containing stem cell extracts for wound repair

[0043] The dressing containing stem cell extracts for wound repair, each 100g of the dressing is composed of the following components and their weight: 1g of induced pluripotent stem cell extract, 5g of chitosan quaternary ammonium salt, 5g of bioactive glass powder, and Jojoba oil 0.3g, glycerin 5.5g and distilled water 82.2g.

[0044] The preparation method of described IPS cell extract is:

[0045] S1. Cultivation of induced pluripotent stem cells: after Matrigel was dissolved overnight at 4°C, dilute Matrigel with DMEM / F12 at a volume ratio of 1:100, mix well, add to a culture dish to coat for 30 minutes, and discard the culture medium , inoculated induced pluripotent stem cells, and incubated at 37°C in CO 2 Cultivated in an incubator;

[0046] S2. Cell extract extraction: suck out the culture medium in the S3 culture bottle, wash it with PBS for 2-3 times, and homogenize it for 3-5 times at b...

Embodiment 3

[0050] Example 3 A dressing containing stem cell extracts for wound repair

[0051] The dressing containing stem cell extracts for wound repair, each 100g of the dressing is composed of the following components and their weight: 10g of induced pluripotent stem cell extract, 1g of chitosan quaternary ammonium salt, 10g of bioactive glass powder, and Jojoba oil 0.5g, glycerin 6.5g and distilled water 65g.

[0052] The preparation method of described IPS cell extract is:

[0053] S2. Cultivation of induced pluripotent stem cells: after Matrigel was dissolved overnight at 4°C, dilute Matrigel with DMEM / F12 at a volume ratio of 1:100, mix well, add to a culture dish to coat for 30 minutes, and discard the culture medium , inoculated induced pluripotent stem cells, and incubated at 37°C in CO 2 Cultivated in an incubator;

[0054] S2. Cell extract extraction: suck out the culture medium in the S3 culture bottle, wash it with PBS for 2-3 times, and homogenize it for 3-5 times at b...