Artificial breeding method of Onychostoma barbatulum
A technology of artificial breeding and shoveljaw fish, which is applied in the field of fish farming, can solve the problems of artificial oxytocin and insemination, heavy workload of oxytocin injection, strong stress response of parent fish, etc., so as to avoid the death of fry and synchronize spawning The effect of strengthening and high survival rate of larvae
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Embodiment 1
[0014] In the last ten days of March, artificially reared third-year-old broodstock were selected. The female fish weighed more than 90g, and the male fish weighed more than 60g. # ,2 # , 3 # , with an area of 30m 2 , the water depth is 40cm~50cm, and the stocking density is 1.5kg / m 2 , The water for breeding is stream water, pH value is 6.8-7.6, dissolved oxygen is above 6mg / L, and the flow rate of pool water is 8h-12h, and the water is changed once. When the water temperature exceeds 15°C, nutrition fortification of broodstock begins, 1 # The spawning pool was fed with an extruded feed with a crude protein content of 35%, and 2 # The spawning pool was fed with an extruded feed with a crude protein content of 42%. # The spawning pool is fed with extruded feed with a crude protein content of 42%+vitamin E+live bait (red earthworm or fly maggot) together, and the amount of vitamin E is 150mg per kg of feed, and the amount of live bait is 100% of the daily feeding amount....
Embodiment 2
[0019] The parent fish selection and test conditions in the early stage were the same as in Example 1. The brood fish in the three spawning ponds were all fed with 42% crude protein content of extruded feed+vitamin E+live bait (red earthworm or fly maggot), aiming at cultivating it well. Beginning in mid-May, 1 # The spawning pool selects female fish with swollen and soft abdomen and red and swollen genital pores for artificial injection of oxytocin. The combination of oxytocin and the injection dose are injected with LRH-A per kilogram of female fish 210 μg + HCG 1000 IU, injected once at the base of the pectoral fins to minimize the damage to the broodstock, and then observe whether the broodstock is chasing after 24 hours of continuous observation. At this time, catch the broodstock and use artificial insemination method to obtain fertilized eggs, but it was found that the spawning rate of female fish was only 10% to 20%, and the mortality rate of broodstock was 30% to 40%....
Embodiment 3
[0021] In terms of hatching methods, two methods of hatching buckets and hatching boxes are compared. The hatching bucket is a 100L white plastic bucket with a cone at the bottom and a cylinder at the top. The bottom is continuously filled with water, and 2,000 fertilized eggs are placed in each liter of water. Next hatch. 8,000-10,000 fertilized eggs are placed in each hatching frame, and placed in an area of 4m 2 ~5m 2 , the water depth is 20cm ~ 30cm, hatched in the hatching tank with shading and micro-flowing water. The other incubation conditions of the two are the same. From the test results, it can be concluded that the hatching rate of the same batch of fertilized eggs in the hatching bucket is only 70% to 80% of that in the hatching box, and after the fertilized eggs hatch out of the membrane, the hatching rate in the hatching bucket The larvae are more likely to die due to the continuous tumbling of the water body. Therefore, the hatching frame method of shading...
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