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Biological control technology for rice sheath blight

A technology for rice sheath blight and biological control, applied in the field of biological control of rice sheath blight, can solve single problems, and achieve the effect of enhancing the control effect and improving the effect of biocontrol

Inactive Publication Date: 2017-11-28
安徽省中日农业环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the single biological control in the prior art, the present invention provides a biological control technology for rice sheath blight, and the mixed use of compounding agents to control rice sheath blight has a synergistic effect

Method used

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Examples

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Embodiment 1

[0025] A biological control technology for rice sheath blight, comprising the following steps:

[0026] Step 1: Prepare NA solid medium, use peptone 10.0g, beef powder 3.0g, sodium chloride 5.0g, agar 15.0g, glucose 2.5g, add distilled water to dissolve, adjust the volume to 1L, adjust the pH to 7±0.1, divide Sterilize at 121°C for 20 minutes after loading;

[0027] Step 2: Prepare LB liquid culture medium, add tryptone 10g, yeast extract 5g, and NaCl 10g in 950ml deionized water, stir until the solute dissolves, adjust the pH to 7.0 with 5mol / L NaOH, and dilute to 1L with deionized water. Steam sterilization under 15psi high pressure for 21min;

[0028] Step 3: Activate the antagonistic bacteria on the NA solid medium at 37°C for 24 hours, make a bacterial suspension with sterile water, take 10 mL of the bacterial suspension and inoculate it into a 250 mL Erlenmeyer flask containing 100 mL of LB liquid culture medium, Shake culture at a rate of 220 r / min for 48 hours at a c...

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Abstract

Relating to the technical field of biological control of rice pests and diseases, the invention provides a biological control technology for rice sheath blight. The technology includes the steps of: preparation of an NA solid medium, preparation of an LB liquid culture solution, preparation of seed liquid, preparation of fermentation liquid, preparation of a fermentation liquid extract, preparation of a mixture, preparation of a duck manure extract, and rice colonization. Mixing of the fermentation extract with validamycin can achieve a synergistic effect on control of rice sheath blight fungi, can significantly enhance the control effect of validamycin on rice sheath blight, provides certain theoretical basis for improving the control effect of validamycin on rice sheath blight, and provides an effective way for expanding the application of antagonistic bacterial biopesticides. The duck manure extract has an inhibiting effect on rice sheath blight fungi, wherein the duck manure ethanol extract has a rice sheath blight pathogen inhibition rate up to 80% or more.

Description

technical field [0001] The invention relates to the technical field of biological control of rice diseases and insect pests, in particular to a biological control technology of rice sheath blight. Background technique [0002] Rice sheath blight is a common disease that affects the growth of rice in my country. Generally, rice sheath blight will cause rice yield reduction by 10%-20%. my country's agricultural production has caused huge economic losses. [0003] However, at present, the effective agent for controlling rice sheath blight is relatively single, mainly Jinggangmycin. Due to long-term use, the rice sheath blight bacteria have developed a certain degree of resistance to Jinggangmycin, and the control effect of a single Jinggangmycin on rice sheath blight has been significantly weakened, making the number of times of use every year gradually increased, and the amount of use is also significant. Increase. Therefore, in order to improve the control effect of rice sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04A01N63/02A01N43/16A01P3/00A01P21/00C12R1/385
CPCA01N43/16A01N63/10C12P1/04C12N1/205C12R2001/385
Inventor 章云张贝尼
Owner 安徽省中日农业环保科技有限公司
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