Compounds, compositions and methods for modulating sweet taste
A composition and compound technology, applied in organic chemistry, food science, etc., can solve problems such as slow sweetness and long-lasting sweetness
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[0396] Preparation of Compounds of the Invention
[0397] In one embodiment, Compound 1 is prepared by the multi-step sequence described below. Those skilled in the art will readily be able to adapt the described conditions for the synthesis of any compound of formula (I).
[0398] 1: Preparation of 5-hydroxy-4-(4-hydroxy-3-methoxyphenyl)-7-methylchroman-2-one
[0399]
[0400] In a 250 mL flask at room temperature, orcinol A (12.4 g, 100.0 mmol) and ferulic acid B (19.4 g, 100.0 mmol) were suspended in 1,4-bis in alkane (100 mL). Concentrated sulfuric acid (98%, 5 mL) was added slowly for 15 minutes. The suspension was heated to 110°C overnight. The dark hot solution was poured into ice water and extracted twice with ethyl acetate / ether (2:1). The combined organic phases were dried over sodium sulfate and evaporated under vacuum to give a solid which was purified with a 330 g column with 0% to 40% ethyl acetate in hexanes to obtain 23.6 g of the two regioisomers Mi...
Embodiment 1
[0408] Example 1: 2 mandatory options for selection
[0409] Effect of Test Compounds on the Perception of Sweetness in High Fructose Corn Syrup (HFCS)
[0410] 2 alternative forced selection methods (2AFC)
[0411] Two alternative forced choice "sip and spit" methods (2AFC) were used to evaluate the effect of test compounds on the perception of sweetness of aqueous solutions of sweeteners in humans.
[0412] Preparation of samples for sensory taste test:
[0413] An aqueous sweetener solution was prepared by adding sucrose equivalent (SE) high fructose corn syrup (HFCS) (by weight) to water to achieve the desired concentration. The pH of the aqueous solution ranges from 2.5 to 7. Compounds were first prepared as 500-fold concentrated stock solutions in 100% ethanol. These concentrated stock solutions were then added to the aqueous sweetener solution to yield a final ethanol concentration of 0.2%. Control (positive and negative) samples were also normalized to 0.2% ...
Embodiment 2
[0421] Example 2: Intrinsic Sweetness
[0422] Intrinsic Sweetness Evaluation
[0423] Preparation of samples for taste testing
[0424] Aqueous sweetener solutions were prepared by adding HFCS (by weight) equivalent amounts of sucrose to water to achieve the desired concentration. The pH of the aqueous solution ranges from 2.5 to 7.
[0425] Compounds were first prepared as 500-fold concentrated stock solutions in 100% ethanol. These concentrated stock solutions were then added to the aqueous sweetener solution to yield a final ethanol concentration of 0.2%. Control (positive and negative) samples were also normalized to 0.2% ethanol. This level of ethanol was not found to contribute to any perceived sweetness.
[0426] Sensory method: Assessing intrinsic sweetness using the 2AFC method
[0427] The intrinsic effect of test compounds in aqueous solution on sweet taste perception in humans was evaluated using the 2AFC method. The test was a double-blind and randomiz...
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