Method for producing cooking wine with high amino acid nitrogen content by using yellow wine stillage
A technology of rice wine distiller's grains and amino acids, which is applied in the field of fermentation, can solve the problems of low amino acid nitrogen, complex distiller's grains treatment, and long enzymatic hydrolysis time, and achieve the effects of improving product quality, reducing production costs, and reducing waste residues
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[0023] Example 1
[0024] A method for producing cooking wine with high amino acid nitrogen content using rice wine lees, such as figure 1 As shown, the rice wine (cooking wine) vinasse is enzymatically hydrolyzed, and the enzymatic hydrolysate is fermented with rice to produce cooking wine. The specific production steps are as follows: 1Kg vinasse is crushed, and 8Kg 50-60℃ hot water is added to soak for 0.5h, and slowly stir. , Blow in 0.2-0.3MPa steam for 10-15min, cool slightly, add 2Kg tap water, wait until the temperature drops to 45-50℃, slowly add sodium hydroxide solution while stirring, adjust its pH to 9.5-10.0.
[0025] Add 5.0×10 5 U alkaline protease, incubate at 45-50°C for 0.5h with constant stirring. This is the enzymatic hydrolysate of vinasse.
[0026] Weigh 100Kg of japonica rice, soak in water (the rice is not over the surface of the water) for 2 days, drain the water, and steam the rice.
[0027] After the rice is steamed, slowly add 70kg of cold boiled water to...
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[0031] Example 2
[0032] 0.5Kg distiller’s grains crushed, add 5Kg 50-60℃ hot water to soak for 0.5h, stir slowly, add 0.2-0.3MPa steam for 10-15min, cool slightly, add 5Kg tap water, wait until the temperature drops to 45-50℃, while stirring While slowly adding sodium hydroxide solution, adjust the pH to 8.5-9.5. Add 4.5×10 4 U alkaline protease, keep it at 40-45°C for 2h with constant stirring. Weigh 100Kg of japonica rice, soak in water (the rice is not over the surface of the water) for 2 days, drain the water, and steam the rice. After the rice is steamed, slowly add 180kg of cold boiled water to the rice, add 10.5Kg of the above-mentioned distiller’s grains enzymatic hydrolysate, add 1kg of wheat koji, 0.1kg of koji, and 0.05Kg of active dry yeast (Koji and active dry yeast need 2.5% glucose solution in advance Activate for 2h), start fermentation after mixing evenly. Control the temperature at 28-32℃ for 0-6 days, stirring occasionally, and control the temperature at 1...
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[0033] Example 3
[0034] 5Kg distiller’s grains are crushed, add 50Kg 50~60℃ hot water to soak for 0.5h, stir slowly, add 0.2-0.3MPa steam for 10-15min, cool slightly, add 50Kg tap water, wait until the temperature drops to 30-40℃, while stirring Slowly add sodium hydroxide solution to adjust the pH to 8.5-10.0. Add 5.0×10 5 U alkaline protease, incubate at 30-35°C for 4.0h with constant stirring. This is the enzymatic hydrolysate of vinasse. Weigh 100Kg of japonica rice, soak in water (the rice is not over the surface of the water) for 2 days, drain the water, and steam the rice. After the rice is steamed, slowly add 36kg of cold boiled water to the rice, add 105Kg of the above-mentioned distillers' grains enzymatic hydrolysate, add 3kg of malt, 0.8kg of koji, and 0.3Kg of active dry yeast (Koji and active dry yeast need to be activated with 2.5% glucose solution in advance 2h), start fermentation after mixing. Control the temperature at 28-32℃ for 0-6 days, stirring occasio...
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