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Tissue culture and rapid propagation method for Davidia involucrata var. vilmoriniana

A technique for tissue culture and rapid propagation of Davidia involucrata, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of seed germination rate that is difficult to collect, and the protection of Davidia glabrata has not been well implemented, and achieves fast reproduction speed , Conducive to recovery, the effect of less separation

Inactive Publication Date: 2017-12-22
贵州省金黔草农业有限公司 +1
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AI Technical Summary

Problems solved by technology

It is extremely difficult to collect seeds and the germination rate of the seeds is often not more than 10% in the conventional use of fruits for propagation, resulting in the protection of Davidia glabrata has not been well implemented

Method used

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Embodiment Construction

[0015] A method for tissue culture and rapid propagation of Davidia glabrata, comprising the following steps:

[0016] A: Acquisition of explants: During September-October in autumn, when the leaves of Davidia glabrata have turned from green to yellow and the leaves have not fallen, cut the annual branches. During the collection process, they must be wrapped in wet newspapers to keep them moist and transported as explant materials. source;

[0017] B: Acquisition of sterile stems: intercept the stems of Davidia glabrata with buds, leave 1.5-2cm stems for the top buds, 0.5cm for the upper end of the side buds, and 1.5-2cm stems for the lower ends, soak in 800 times carbendazim for 20min, and then drip Add 2 drops of Tween, 2-3 drops of detergent, stir well, after cleaning, cover the mouth of the container with gauze, and rinse it under running water for 2 hours; put the rinsed material on the ultra-clean workbench, first use 75 % alcohol for 30 seconds, and then directly put t...

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PUM

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Abstract

The invention provides a tissue culture and rapid propagation method for Davidia involucrata var. vilmoriniana. The tissue culture and rapid propagation method comprises the following steps: A, acquisition of explant; B, acquisition of a sterile stem section; C, germination induction of lateral buds; and D, induction and multiplication of multiple shoots. Compared with traditional methods for propagation via seed sowing, the method provided by the invention has the following substantial characteristics: propagation speed is fast, and propagation of seedlings from explant only takes 4 months; a propagation coefficient is high and seedlings grow exponentially; seedling propagation can be carried out all the year round without influence by external environment; seedlings are good in consistency, and the situation of segregation hardly occurs; all the seedlings are virus-free seedlings, which is beneficial for improving the resistance of Davidia involucrata var. vilmoriniana and reducing diseases and pest attack; investment is low, high economic benefits are obtained, large-scale propagation can be carried out, and bottlenecks in a seedling source are overcome; and cultivation of tissue culture plants in a wild environment is beneficial for restoration of a wild population, and good economic, social and ecological benefits are produced.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of Davidia glabrata, belonging to the field of biotechnology. Background technique [0002] Davidia glabrata ( Davidia involucrata Baill. var. vilmoriniana) is a deciduous tree unique to my country. There are two species, one is Davidia involucrata and the other is Davidia glabrata. The two are similar, the difference lies in whether the leaves are hairy or not. It is a relict plant left by the Cenozoic Tertiary period 10 million years ago. Affected by the Quaternary glacial period, the Davidia involucrata populations in most areas have been extinct one after another, and now they are only scattered in Sichuan, Guizhou, Hubei and other places in my country. , has been included in the "Chinese Plant Red Book" and "China Rare and Endangered Plant List", and has been listed as a national first-level key protected wild plant. [0003] The flowers and branches of Davidia glabrata a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 陈杰廖冬云李涛徐庆祝李恒谦韦利霞周林涛张婷婷张翔宇
Owner 贵州省金黔草农业有限公司
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