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Application of vipr1 gene in the preparation of liver cancer diagnostic products and prognosis prediction products

A liver cancer prognosis and liver cancer technology, applied in the field of biomedicine, to achieve the effect of reducing mortality and accurately predicting the prognosis of liver cancer

Inactive Publication Date: 2020-09-11
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the correlation between VIPR1 and the occurrence and prognosis of liver cancer has not been reported yet.

Method used

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  • Application of vipr1 gene in the preparation of liver cancer diagnostic products and prognosis prediction products
  • Application of vipr1 gene in the preparation of liver cancer diagnostic products and prognosis prediction products
  • Application of vipr1 gene in the preparation of liver cancer diagnostic products and prognosis prediction products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0047] Example 2 Fluorescent quantitative PCR verification of the relationship between VIPR1 and liver cancer

[0048] 2.1 Organization source

[0049] Thirty cases of liver cancer and its paracancerous tissues were collected from the Department of Hepatobiliary Surgery of the Affiliated Hospital of Guilin Medical College from January 2015 to September 2015.

[0050] 2.2 Fluorescent quantitative PCR detection

[0051] 2.2.1 Extraction of total RNA from liver cancer tissue

[0052] Take liver cancer tissue frozen and stored in liquid nitrogen, grind it in a pre-cooled mortar, add 1mL Trizol after the tissue sample is powdered, let it stand at room temperature for 5min, transfer the lysate into a 1.5mL centrifuge tube, add 0.2mL chloroform, and shake vigorously Centrifuge tubes and place at room temperature for 10 minutes. After centrifugation at 12000rpm for 15min, take the upper aqueous phase into another new centrifuge tube. Add an equal volume of pre-cooled isopropanol, ...

Embodiment 3

[0059] Example 3 Immunohistochemical verification of the relationship between VIPR1 and liver cancer

[0060] 3.1 Organization source

[0061] Liver cancer (HCC) tissue chips were purchased from Shanghai Xinchao Biotechnology Co., Ltd. The HCC tissue chip (HLiv-HCC180Sur-05) contained 95 HCC tissues and 85 paired paracancerous tissues. The operation period was from August 2006 to September 2011, and the follow-up period ended in September 2013. Follow-up records were missing in 10 cases.

[0062] 3.2 Immunohistochemical (IHC) staining

[0063] Put the tissue chip into an oven, adjust the temperature to 63°C, and bake the wax for 1 hour. After the film is baked, it is taken out from the oven and placed in an automatic dyeing machine for dewaxing. Take out the slice from the dyeing machine, rinse with pure water 3 times, 2min each time. During the rinsing process, put the citric acid repair solution on the induction cooker and start heating; after the citric acid repair so...

Embodiment 4

[0068] Example 4 The expression of VIPR1 in liver cancer tissue is related to the clinical prognosis of patients

[0069] The clinicopathological characteristics of the 85 patients in the whole group were statistically analyzed and found that the expression of VIPR1 was related to the histopathological grade of the patients. The results are shown in Table 1.

[0070] Table 1 The relationship between the expression level of VIPR1 and the clinicopathological characteristics of patients

[0071]

[0072] Survival analysis found that the survival rate of patients with high expression of VIPR1 in liver cancer tissues was higher (P=0.0055). The result is as Figure 13 shown. For the overall survival rate, univariate analysis showed that high VIPR1 expression, tumor size ≤ 5cm, high / moderate differentiation and lower TNM stage were associated with higher overall survival rate, multivariate analysis using Cox proportional hazards regression model, the results showed The express...

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Abstract

The invention discloses applications of a VIPR1 gene in preparing liver cancer diagnosis products and prognostic prediction products, and belongs to the technical field of bio-medicine. The invention discloses an application of the VIPR1 gene in preparing the liver cancer diagnosis products, and also discloses an application of the VIPR1 gene in preparing the liver cancer prognostic prediction products as well as an application of the VIPR1 gene in preparing liver cancer treating medicines. According to the invention, it is discovered for the first time that the VIPR1 is in abnormal expression in solid tumor liver tissues, and it is discovered that the VIPR1 gene can serve as a specific marker gene for liver cancer diagnosis and liver cancer prognostic prediction; in comparison with conventional detection means, the VIPR1 gene is rapider, more specific, more sensitive and more accurate; the early diagnosis and prognostic prediction of liver cancer can be achieved, so that the death rate of the liver cancer can be reduced; and a novel treatment target and a novel effective medicine are provided for the prevention and treatment of the liver cancer.

Description

technical field [0001] The invention relates to the application of VIPR1 gene in the preparation of liver cancer diagnosis products and prognosis prediction products, and belongs to the technical field of biomedicine. Background technique [0002] Liver cancer is the second leading cause of cancer death in men globally and in less developed countries. The incidence of liver cancer is highest in East and Southeast Asia and North and West Africa, and lowest in Central and South Asia and Northern Europe, and Central and Eastern Europe. About 50% of new cases and deaths are in China. Because the early symptoms of liver cancer are not obvious, patients are often in the middle and late stage when they are discovered, and the chance of surgery is lost. In addition, liver cancer is a highly invasive cancer. Tumor invasion, intrahepatic spread and extrahepatic metastasis are the main causes of death of patients. Despite advances in diagnostic and therapeutic techniques, the prognosi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/574
CPCC12Q1/6886C12Q2600/118C12Q2600/158G01N33/57438G01N33/57484G01N2800/52
Inventor 莫之婧金荣仲卢思聪李康智马义丽
Owner GUILIN MEDICAL UNIVERSITY