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Composition for treating ovine mycoplasma pneumonia
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A technology of mycoplasma ovinee and a composition is applied in the field of compositions for treating mycoplasma ovinee pneumonia, and can solve problems such as reduced disease resistance and the like
Active Publication Date: 2020-09-04
SHIHEZI UNIVERSITY
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However, studies have reported that the hybrid offspring of Argali and Bashibai sheep have good production performance, but their disease resistance is greatly reduced, and they are susceptible to Mycoplasma ovipneumoniae (Mycoplasma ovipneumoniae, MO)
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Embodiment 1
[0038] This embodiment provides a composition for the treatment of Mycoplasma ovis pneumonia and its treatment method.
[0039] 1 Materials and methods
[0040] 1.1 Proteins, strains and laboratory animals
[0041] The recombinant SPLUNC1 protein of Bashbai sheep and argali hybrid sheep was expressed by Chen Kaili and Gao Baode in our laboratory with Pichia pastoris GS115 / pPIC9K-SPLUNC1, and the expression product was purified by nickel column chromatography; MO strain was expressed by the School of Animal Science and Technology, Shihezi University Provided by Professor Yan Genqiang from the Preventive Veterinary Laboratory, with 98% homology with the Y98 standard strain; 18 argali hybrid sheep and 6 Bashbai sheep were provided by Yumin County, Xinjiang, all 2 to 3 months old, weight 10-15kg, MO ELISA antibody test results are all negative.
[0085] In this example, the test sheep are 24 argali hybrid sheep, and the method of artificially infecting MO is the same as in Example 1.
[0086] The 24 argali hybrid sheep were randomly divided into three groups A, B, C, and D. The 24 hybrid sheep were artificially infected with MO. After testing, the 24 argali hybrid sheep were all infected with MO.
[0087] On the 5th day after infection, group A was injected with recombinant Bashbai sheep SPLUNC1 protein once a day in the trachea, group B was injected with recombinant argali hybrid sheep SPLUNC1 protein, 1mL / head (concentration 150μg / mL), group C was injected with recombinant Bash into the trachea The mixture of Baiyang SPLUNC1 protein and recombinant argali hybrid sheep SPLUNC1 protein, 1mL / head (concentration 150μg / mL, buffer is PBS buffer). In group D, an equal amount of normal saline was injected into the trachea, once a day.
[0088] On the 7th day of the injection, the clinical symptoms of the experimental sheep in grou...
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Abstract
The invention relates to a composition for treating mycoplasma pneumonia of sheep and a treatment method thereof. The composition includes first protein or second protein, wherein the first protein isrecombined SPLUNC1 protein of Bashbay sheep, the second protein is recombined SPLUNC1 protein of argali and hybridized sheep, and the composition is used for treatment of treatign mycoplasma pneumonia of the sheep. The treatment method of treatign mycoplasma pneumonia of the sheep is simple to operate, obvious in treatment effect and capable of increasing the survival rate of hybridized sheep ofargali and Bashbay sheep.
Description
Technical field [0001] The invention relates to a composition, in particular to a composition for the treatment of Mycoplasma ovis pneumonia and a treatment method thereof. Background technique [0002] Argali is a second-level national protected wild animal with a large body and strong disease resistance; Bashbai sheep is a characteristic breed of Xinjiang with strong disease resistance and resistance to Mycoplasma ovipneumoniae (MO) infection . Studies have reported that the hybrid offspring of argali and Bashbai sheep have good production performance, but their disease resistance is greatly reduced, and they are susceptible to Mycoplasma ovipneumoniae (Mycoplasma ovipneumoniae, MO). [0003] Short palate, lung and nasal epithelium clone 1 (SPLUNC1) protein is a secreted protein, which is highly expressed in airway epithelium. Studies have shown that the use of gene knockout methods to establish a SPLUNC1 gene deletion mouse model. After 24 hours of Mycoplasma (Mycoplasma pneum...
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