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Evaluation method for rat model with glomerulosclerosis

A glomerulosclerosis, rat model technology, applied in the direction of instruments, complex mathematical operations, measuring devices, etc., can solve problems such as low accuracy, high cost, time-consuming and laborious, and achieve rationality, scientificity, and specificity. strong effect

Inactive Publication Date: 2018-01-09
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the technical problems of low accuracy, high cost and time-consuming and labor-intensive evaluation methods of existing glomerular sclerosis models, and to provide an evaluation method for glomerular rat models

Method used

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  • Evaluation method for rat model with glomerulosclerosis
  • Evaluation method for rat model with glomerulosclerosis
  • Evaluation method for rat model with glomerulosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Twelve male SD rats were randomly divided into a normal control group (sham operation group) and a model group (8 rats in each group). The experiment began after one week of adaptive feeding in an SPF animal room, with free access to food, water and natural light. The rat model of glomerulosclerosis was constructed by left nephrectomy combined with doxorubicin hydrochloride injection into the rat tail vein. The specific method is: use 10% chloral hydrate (by 0.3ml·100g -1Conversion) Anesthetized rats were injected intraperitoneally, fixed in supine position, skin was prepared on the abdomen, disinfected with povidone iodine, under sterile conditions, a longitudinal incision of about 3 cm was made along the left side of the abdominal midline at 1 cm, the skin and tissue layers were separated layer by layer, and the left side was exposed. For the kidney, use No. 4-0 surgical suture to ligate the renal artery and renal vein together near the renal hilus, and then perform a...

Embodiment 2

[0040] [Example 2] Establishment of an evaluation method for a rat model of glomerulosclerosis

[0041] 1) The metabolic profile was characterized by multivariate statistical analysis method, and the pattern recognition of the data was carried out by principal component analysis (PCA), and the separation of the data profiles of each group was investigated. The specific method is to collect the urine of the rat model on the 7th day, the 10th day and the 14th day after the operation of the rat, and first collect the urine of the rat model on the 7th day, the 10th day and the 14th day after the operation. The liquid was analyzed by LC-MS respectively, and the LC-MS spectrum of the rat model was obtained; then, the multivariate statistical analysis was performed on the LC-MS spectrum integration data matrix of the rat model, and the contour map of the rat model was obtained, and then the rat model was analyzed. The outline diagram of the model is analyzed dynamically to obtain the...

Embodiment 3

[0064] The samples in this example are all from the rats in Example 1 (6 rats in the normal control group and 6 in the model group). On the basis of using the evaluation method obtained in Example 2 to verify the successfully constructed glomerulosclerosis model, the following tests and analyzes were continued.

[0065] 1. Detection of biochemical indicators

[0066] Table 1 Changes in 24h urinary protein content of rats in each group on day 0, 7, 10, and 14 after operation (Mean±SD)

[0067]

[0068] Compared with normal control group, *P<0.05; **P<0.01

[0069] Compared with the control group, the amount of urine protein in the model group rats on the 7th day, 10th day, and 14th day after operation was significantly higher than that of the control group (P<0.01). (shown in Table 1)

[0070] The reliability of the model was evaluated by the changes of the biochemical indicators of the two groups of rats before and after modeling:

[0071] Table 2 Changes of TP, ALB, GL...

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Abstract

The invention discloses an evaluation method for a rat model with glomerulosclerosis. The evaluation method comprises steps as follows: firstly, urine of the rat model is collected on day 0, on the seventh day, on the tenth day and on the fourteenth day and subjected to LC-MS (liquid chromatography-mass spectrometry) determination and analysis respectively, and an LC-MS spectrogram of the rat model is obtained; an integral data matrix of the LC-MS spectrogram of the rat model is subjected to multivariate statistical analysis, and a profile diagram of the rat model is obtained; the profile diagram of the rat model is subjected to dynamic profile analysis, a dynamic profile change trend diagram of the rat model is obtained, and degree of deviation on the fourteenth day of model constructionis the highest compared with that on day 0; analysis based on Marker-view software indicates that the construction of the rat model with glomerulosclerosis succeeds when content of 15 biomarkers on the fourteenth day of model construction meets the requirement compared with that on day 0; the technical problem that an existing construction and evaluation method for the rat model with glomerulosclerosis is low in accuracy, high in cost, time-consuming and labor-consuming is solved.

Description

technical field [0001] The invention belongs to the technical field of evaluation methods for rat models, and in particular relates to an evaluation method for rat models of glomerulosclerosis. Background technique [0002] Since kidney disease and urinary tract disease were included in the 15 diseases with the highest global fatality rate, chronic kidney disease (CKD) has been widely regarded as a major disease threatening public health worldwide. Pathological studies have shown that glomerulosclerosis is a sign of CKD developing into chronic renal failure (CRF), and it is also a common result of CKD developing into nephrotic syndrome (focal segmental glomerulosclerosis, FSGS). At present, modern medicine still lacks effective treatment methods for glomerulosclerosis. Once patients develop the disease, it is difficult to cure, which seriously threatens the quality of life of human beings. [0003] In recent years, with the continuous deepening of research in the field of g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G06F17/16
Inventor 周本宏陈鹏涂杰陈富超邱振鹏
Owner WUHAN UNIV
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