An indel marker closely linked to the green peach aphid resistance trait of peach germplasm and its application
A pp-indel-23, marker technology, applied in the field of molecular breeding, can solve problems such as poor pesticide effect and aphid variation, and achieve the effect of saving land
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] Example 1: Screening of aphid-resistant plants from the offspring population of peach germplasm 2013-04-20R
[0020] 1. Collect individual leaves from 2013-04-20R offspring, and extract leaf DNA with a plant DNA extraction kit; dilute the DNA to 20ng / ul;
[0021] 2. Select the detection site molecular marker primers Pp-InDel-23F and Pp-InDel-23R in the invention, and amplify the molecular markers by PCR. The PCR reaction system is: total volume 20μl, 1×PCR buffer, 1.5mmol / L MgCl2, 0.2mmol / L dNTPS, 0.4mmol / L primers Pp-InDel-23F and Pp-InDel-23R, 25ng template DNA, 1U Tag Enzyme; PCR reaction conditions: Denaturation at 95°C for 3min, followed by 35 cycles at 94°C for 30s, 53°C for 25s, 72°C for 15s, and finally extension at 72°C for 5min.
[0022] 3. After the amplified product is separated by electrophoresis on a 6% polyacrylamide gel, detect the band type to see if it is a heterozygous band type or contains a 93bp resistant band (attached figure 1 ). The 6% polyac...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com