A group of InDel sites closely linked with mountain peach aphid resistance main-effect QTL qGPAR-3-1 and application thereof
A site and mountain peach technology, applied in the fields of peach aphid-resistant molecular breeding and germplasm resource utilization, can solve the problems of many environmental impact factors, difficult aggregation, low efficiency, etc., and achieve the effect of saving land
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[0025] Example 1 Screening of Aphid-resistant Plants of Prunus officinale Progeny Population
[0026] 1. Collect individual leaves from the offspring of Prunus chinensis, and extract DNA from the leaves with a plant DNA extraction kit; dilute the DNA to 20ng / ul;
[0027] 2. Select the molecular marker primer corresponding to any site in the invention, and amplify its molecular marker by PCR, and select the InDel25 marker primer here. The PCR reaction system is: total volume 20μl, 1×PCR buffer, 1.5mmol / L MgCl2, 0.2mmol / L dNTPS, 0.4mmol / L primer InDel25-F and primer InDel25-R, 25ng template DNA, 1U Tag enzyme; Reaction conditions: Denaturation at 95°C for 3min, followed by 35 cycles at 94°C for 30s, 55°C for 45s, 72°C for 20s, and finally extension at 72°C for 5min.
[0028] 3. After the amplified product is separated by electrophoresis on a 6% polyacrylamide gel, check whether it contains an InDel25 resistance molecular marker with a size of 168bp (attached figure 1 ). The 6...
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