InDel mark tightly linked with wild peach germplasm anti-green-peach-aphid properties and application thereof

A pp-indel-23, marker technology, applied in the field of molecular breeding, can solve problems such as poor pesticide effect and aphid variation, and achieve the effect of saving land

Active Publication Date: 2018-01-19
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

In recent years, the effectiveness of pesticides such as thiamethoxam and imidacloprid in the control of aphids has gradually deteriorated, which is related to the resistance of aphids to mutations.

Method used

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  • InDel mark tightly linked with wild peach germplasm anti-green-peach-aphid properties and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Screening of aphid-resistant plants from the offspring population of peach germplasm 2013-04-20R

[0020] 1. Collect individual leaves from 2013-04-20R offspring, and extract leaf DNA with a plant DNA extraction kit; dilute the DNA to 20ng / ul;

[0021] 2. Select the detection site molecular marker primers Pp-InDel-23F and Pp-InDel-23R in the invention, and amplify the molecular markers by PCR. The PCR reaction system is: total volume 20μl, 1×PCR buffer, 1.5mmol / L MgCl2, 0.2mmol / LdNTPS, 0.4mmol / L primers Pp-InDel-23F and Pp-InDel-23R, 25ng template DNA, 1U Tag enzyme ;PCR reaction conditions: Denaturation at 95°C for 3min, followed by 35 cycles at 94°C for 30s, 53°C for 25s, 72°C for 15s, and finally extension at 72°C for 5min.

[0022] 3. After the amplified product is separated by electrophoresis on a 6% polyacrylamide gel, detect the band type to see if it is a heterozygous band type or contains a 93bp resistant band (attached figure 1 ). The 6% polyacr...

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Abstract

The invention discloses an InDel mark tightly linked with the wild peach germplasm anti-green-peach-aphid properties and application thereof. An InDel site detected by a molecular marker Pp-InDel-23 corresponds to the 44057689th basic group position of the Lovell genome assembled Scaffold 1; the basic group sequence in the position is A or AGTCAAAT; the mark with the basic group in the site position being A is a resistant mark. The site and molecular marker method disclosed by the invention has the advantages that the detection site and an efficient tool can be provided for the peach anti-green-peach-aphid molecule breeding, anti-aphid property multi-property polymerization breeding and anti-aphid resource screening; the application prospects are wild.

Description

technical field [0001] The invention relates to germplasm resistant green peach aphid ( Myzus persicae ) The closely linked InDel marker and its application method belong to the field of molecular breeding, and are specially used for peach aphid-resistant molecular breeding and germplasm resource screening and utilization. Background technique [0002] Peach is native to China and has rich genetic diversity. China's peach cultivation area and output both account for more than 50% of the world, ranking first in the world. The green peach aphid belongs to the Hemiptera Aphididae, sucks the phloem sap of plants through a stylet, and is one of the main pests of peach trees in spring. Many stone fruit viroids can be transmitted by aphids. At present, the control of peach tree aphids mainly relies on chemical pesticides. During the use of chemical pesticides, it is inevitable to kill a large number of agricultural beneficial insects and destroy biodiversity. Affected by clima...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 王新卫王力荣曹珂陈昌文方伟超朱更瑞薛梅真
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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