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P2Y12 receptor platelet aggregation detection reagent card and production method thereof

A technology of P2Y12 and platelet aggregation, applied in the field of medical materials, achieves the effect of simple operation, rapid detection and high reliability

Inactive Publication Date: 2018-01-19
北京乐普诊断科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no reports about the use of optical turbidimetry to quickly detect the platelet aggregation function of patients in China. Therefore, it is expected to develop a detection reagent card that can quickly and effectively detect the platelet aggregation function of patients in this field, so as to serve clinicians and doctors alike. Patients provide useful auxiliary data for predicting the risk of future thrombosis or bleeding in patients receiving P2Y12 receptor antagonist therapy

Method used

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  • P2Y12 receptor platelet aggregation detection reagent card and production method thereof
  • P2Y12 receptor platelet aggregation detection reagent card and production method thereof
  • P2Y12 receptor platelet aggregation detection reagent card and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) In this example, the microsphere material, particle size, functional functional group, GP IIb / IIIa receptor ligand protein, near-infrared dye, and iso-TRAP / ADP / PGE1 activator that act as a carrier are determined Dosage and components of the lyoprotectant solution, the above is microsphere labeling, preparation of lyophilized reagents.

[0047] (2) Utilize the raw material composition and consumption determined in this embodiment to prepare freeze-dried reagents, the preparation method is as follows:

[0048] Microsphere labeling:

[0049] ①Microsphere cleaning: take 1mL of carboxyl microspheres, wash twice with 6mL 0.02M pH5.0 phosphate buffer, centrifuge at 2000g at 4°C (2000g, 5min) and discard the supernatant.

[0050] ②Microsphere activation: Add EDC and NHS solutions prepared with 0.02M pH5.0 phosphate buffer to a final concentration of 20mg / mL, activate at room temperature for 25 minutes, centrifuge at 4°C (2000g, 5min), discard the supernatant, and wash with...

Embodiment 2

[0063] Using the P2Y12 receptor platelet aggregation detection reagent card prepared in Example 1 to detect platelet aggregation function, the method is as follows:

[0064] P2Y12 receptor platelet aggregation detection reagent card is a transparent reagent card made of polystyrene material, which contains four detection channels, one channel contains freeze-dried reagents coated with BSA and fibrinogen, no activator, two Channels contain freeze-dried reagents coated with BSA and fibrinogen, and platelet maximum activator iso-TRAP (thrombin receptor activating peptide), three channels do not contain freeze-dried reagents, as a blank control, four channels contain Freeze-dried reagent coated with BSA and fibrinogen, containing platelet P2Y12 receptor activator ADP (adenosine diphosphate) and P2Y1 receptor antagonist PGE1 (prostaglandin E1).

[0065] Label the four detection channels of the P2Y12 receptor platelet aggregation detection reagent card, such as figure 1 shown.

[...

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Abstract

The invention provides a P2Y12 receptor platelet aggregation detection reagent card and a production method thereof. The P2Y12 receptor platelet aggregation detection reagent card comprises four detection channels, the first channel contains a lyophilized reagent coated with BSA and fibrinogen and contains no activators, the second channel contains a lyophilized reagent coated with the BSA and fibrinogen and also contains a platelet maximum activator iso-TRAP (thrombin receptor activating peptide), the third channel contains no lyophilized reagents, and the fourth channel contains the lyophilized reagent coated with the BSA and fibrinogen, and also contains a platelet P2Y12 receptor activator ADP (adenosine diphosphate) and a P2Y1 receptor antagonist PGE1 (prostaglandin E1). The patient'sP2Y12 receptor pathway-correlated platelet aggregation function is rapidly and effectively detected through optical nephelometry by using the detection reagent card. The card has the advantages of high sensitivity, strong specificity, easiness in operation, and suitableness for bedside fast detection, and can well predict the future thrombus or bleeding risk in patients treated with the P2Y12 receptor antagonists.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and relates to a preparation method of a P2Y12 receptor platelet aggregation detection reagent card and an application thereof. Background technique [0002] Platelets play a key role in maintaining normal hemostasis. When exposed to damaged blood vessels, platelets will adhere to the exposed subendothelial matrix, and subsequently, various factors (such as thrombin, ADP, or collagen, etc.) released or produced at the damaged site activate platelets. After platelet activation, the glycoprotein GPIIb / IIIa receptor on the platelet undergoes a conformational change, allowing it to bind to multivalent fibrinogen, causing adjacent platelets to recruit to the injury site and aggregate to form a hemostatic plug or thrombus. [0003] Platelet aggregation in vitro depends on the activation of platelets by aggregation inducers (thrombin, adenosine diphosphate (ADP)), which binds to fibrinogen co...

Claims

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Application Information

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IPC IPC(8): G01N33/86
Inventor 郎天丹李阳魏明明邱笑违董飒英
Owner 北京乐普诊断科技股份有限公司
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