Preparation method of animal model with oral ulcer caused by fire excess from yin deficiency

A technology of yin-deficiency-fire-excessive type and production method, which is applied to preparations for in vivo tests, pharmaceutical formulas, active ingredients of hydroxyl compounds, etc., can solve problems such as no animal models of yin-deficiency and fire-excessive oral ulcers have been found, and achieve great application value , high applicability, less difficult to achieve the effect

Pending Publication Date: 2018-01-26
SUN YAT SEN UNIV
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Problems solved by technology

However, no literature has been found on the production of oral

Method used

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  • Preparation method of animal model with oral ulcer caused by fire excess from yin deficiency
  • Preparation method of animal model with oral ulcer caused by fire excess from yin deficiency
  • Preparation method of animal model with oral ulcer caused by fire excess from yin deficiency

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Embodiment

[0015] Example: Production of Animal Model of Oral Ulcer of Yin Deficiency and Exuberance of Fire

[0016] 1. Materials

[0017] (1) Animals: 24 SD male rats, SPF grade, body weight 120-150 g, provided by Guangdong Medical Experimental Animal Center, license number: SCXK (Guangdong) 2013-0002.

[0018] (2) Instruments and reagents: anesthesia (MATRX), CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd., KQ-250DE), electronic analytical balance (Sartorius), electronic thermometer (OMRON MC-347), refrigerator (Siemens), plastic Thin tube (length 6cm, inner diameter about 5mm); distilled water, normal saline, triiodothyronine, phenol, chloral hydrate, isoflurane, 0.1mol / L sodium hydroxide solution, BCA protein concentration determination kit (Bi Yuntian Biotechnology Research Institute), superoxide dismutase (SOD) test kit, nitric oxide synthase (NOS) test kit, malondialdehyde (MDA) assay kit, glutathione-peroxidase (GSH -Px) test box (Nanjing Jiancheng Institute of...

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Abstract

The invention discloses a preparation method of an animal model with oral ulcer caused by fire excess from yin deficiency. The method comprises selecting healthy rats, carrying out subcutaneous injection on the rats with a triiodothyronine solution according to the dosage of 5-200 micrograms per kilogram for 14-21 days, carrying out impregnation on a cotton ball with phenol, and firing the rat lateral buccal mucosa to form a white wound so that a desired animal model can be obtained after 24-48h. Compared with the prior art, the method is used for preparation of an animal model with oral ulcercaused by fire excess from yin deficiency. The model can induce the oral ulcer based on the fire excess from yin deficiency according to the traditional Chinese medicine differentiation of symptoms and signs, well satisfies clinical practice, has high feasibility and high applicability and has a great application value in heat and fire removal, tissue damage reduction and ulcer healing promotion.

Description

technical field [0001] The invention belongs to the field of Chinese patent medicines, and in particular relates to a preparation method of an animal model of oral ulcer of the type of yin deficiency and hyperactivity of fire. Background technique [0002] Today, people's work and life pressure is increasing, irregular work and rest, unreasonable diet, and environmental pollution make oral inflammatory disease a common and frequently occurring disease. In clinical practice, oral inflammatory diseases can also be induced by other diseases or caused by disease treatment. There are many types of oral inflammatory diseases, including recurrent oral ulcers, oral mucositis, oral lichen planus, and thrush. Oral pain, inconvenient eating, and repeated attacks are common in patients. At the same time, it may lead to various complications in the body and seriously affect their health. Therefore, it is necessary to effectively treat oral inflammatory diseases. [0003] Traditional C...

Claims

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Application Information

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IPC IPC(8): A61K31/198A61K31/05A61K49/00
Inventor 苏薇薇刘宏郑艳芳吴忠李沛波王永刚彭维
Owner SUN YAT SEN UNIV
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