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Method for breeding high-yield enramycin strain

A technology of enramycin and high-yield strains, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and electric/wave energy processing enzymes, can solve the problems of complex metabolic regulation mechanisms of antibiotics, and solve the problems of continuous degradation, overcome blindness effect

Inactive Publication Date: 2018-01-26
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

However, for most antibiotic-producing bacteria, because the biosynthesis mechanism and metabolic regulation mechanism of antibiotics are very complex, the production of antibiotics is often regulated by multiple factors, so it is difficult to achieve the desired effect through simple genetic manipulation

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  • Method for breeding high-yield enramycin strain
  • Method for breeding high-yield enramycin strain
  • Method for breeding high-yield enramycin strain

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Embodiment Construction

[0034] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0035]The invention provides a screening method for high-yield strains of enramycin (Enduracidin), which introduces an antibiotic resistance marker gene (thiostrepton resistance gene tsr) into the enramycin producing bacteria, and makes it It is linked with the key gene (short plate gene) endD in the enramycin biosynthesis gene cluster, so that the expression level of tsr gene is positively correlated with the expression level of endD gene, because the endD gene is the key to determine the production of enramycin (short plate gene ) gene, so high enramycin-producing strains can be obtained by screening highly resistant thiostrepton strains. The invention adds a screening marker for the screening of enramycin high-yield strains (mutant strain...

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Abstract

The invention relates to a method for breeding a high-yield enramycin strain. The method comprises the following steps: determining the short gene of a rate-limiting enzyme coding gene in the enramycin biological synthesizing process; constructing a thiostrepton resistance gene integrating vector; mutagenizing the modified strains, and screening a thiostrepton high-resistance strain from the modified strains; and carrying out genetic manipulation on the screened high-resistance strain, and restoring the tsr open reading frame to the original endX gene to obtain the strain which is an enramycinhigh-yield mutant strain. The method combines advantages of a traditional mutation breeding technology and a modern molecular biological method to realize quick and efficient screening of the enramycin high-yield strain. The method is applicable to screening of other antibiotic high-yield strains.

Description

technical field [0001] The invention relates to a breeding method for obtaining high-yielding enramycin strains, which belongs to the technical field of using streptomyces to increase antibiotic production, and specifically relates to the breeding of high-yielding strains, the construction of intermediate carriers, the use of resistance screening and replenishment Genes to obtain high-yielding strains. Background technique [0002] Enramycin, also known as persistent mycin, is a non-ribosomal polypeptide (NRP) antibiotic produced by the fermentation of actinomycetes in the soil, which is harmful to Gram-positive bacteria, especially in the intestinal tract of poultry and livestock Bacillus spp. has a strong inhibitory effect; adding an appropriate amount of enramycin to the feed can not only prevent common animal digestive tract diseases, but also improve the community balance in the intestinal tract of animals, which is beneficial to the digestion and absorption of feed nut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/76C12N15/65C12N15/01C12N13/00C12R1/01
Inventor 路福平张会图宋淑婷
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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