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Method for establishment of macaca mulatta liver fibrosis model by intraperitoneal injection

A technique for intraperitoneal injection and liver fibrosis, which is applied in pharmaceutical formulations, active ingredients of halogenated hydrocarbons, additional food elements, etc., can solve the problems of difficult modeling and correspondence, and achieves the effect of high success rate, high efficiency and simple method.

Active Publication Date: 2018-02-16
SICHUAN PROVINCIAL PEOPLES HOSPITAL
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  • Abstract
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Problems solved by technology

[0003] At present, the widely used animal models of liver fibrosis include chemical liver fibrosis model, alcoholic liver fibrosis model, immune liver fibrosis model, bile duct obstruction liver fibrosis model and compound factor liver fibrosis model. Humans and other commonly used small animal models are very mature and convenient animal models, but due to the large genetic background and physiological gap between mice and other small animals and humans, it is difficult to directly compare the models constructed by them with human diseases. correspond

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  • Method for establishment of macaca mulatta liver fibrosis model by intraperitoneal injection
  • Method for establishment of macaca mulatta liver fibrosis model by intraperitoneal injection

Examples

Experimental program
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Embodiment 1

[0018] Select 10 male rhesus monkeys aged 2-3 years and weighing 5-9kg as model animals. During the modeling process, they were fed with conventional feed for 15 days at first, and the drinking water was normal tap water. After 15 days, the conventional feed was changed to For high-fat feed, the drinking water is 10% alcoholic water solution (absolute ethanol mixed with purified water) for drinking water, and injects CCl twice a week to each rhesus monkey. 4 Injection, the volume of each injection is 1.0mL / kg, and the injection method is intraperitoneal injection. After 20 injections (10 weeks), pathological biopsy showed fatty changes; for 30 weeks, rhesus monkeys showed moderate fibrosis symptoms .

[0019] Wherein, the high-fat feed includes the following components in weight percentage: 16.78% crude protein, 21.35% crude fat, 10% water, 6.8% ash, 3.2% crude fiber, 0.92% calcium, 0.71% phosphorus, and 0.32% cholesterol.

[0020] The 10% alcohol aqueous solution is prepared...

experiment example

[0024] Experimental example Evaluation method and results of rhesus monkey liver injury diagnosis model

[0025] 1. Detection of serum biochemical markers

[0026] Serum biochemical markers mainly include alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), bilirubin (BIL), serum total cholesterol (TCH), triglyceride (TG), clotting time (PT and creatinine (Cr).

[0027] After 10 and 20 weeks of modeling, the experimental rhesus monkeys were fasted for 12 hours, and then injected with 0.5 mL of Sutai for intramuscular anesthesia, 5 mL of blood was collected from the vein of the hind limb, centrifuged at 3000 r / min for 15 minutes, the serum was separated, and the content of biochemical markers in the serum was tested , it was found that the content of each marker was significantly different from that of the control group (P<0.05); the control group was fed with conventional feed and drank tap water throughout the process.

[0028] 2. Histopathologic...

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Abstract

The invention provides a method for establishment of a macaca mulatta liver fibrosis model by intraperitoneal injection. The method includes the steps of: selecting 5-9kg male macaca mulattas of 2-3 years old as the model animals, performing feeding with high-fat feed during modeling, supplying an alcohol water solution with a volume concentration of 9%-11% for drinking, and injecting CCl4 injection to each macaca mulatta during modeling twice a week by intraperitoneal injection continuously for 20-30 weeks, and controlling the injecting amount at 0.8-1.5mL / kg each time, thus achieving successful modeling. The animal model establishment method provided by the invention is simple, safe and reliable, has the characteristics of short modeling time, high success rate, good repeatability, goodanimal tolerance and low death rate, and can be used for studying the pathogenesis, pathogenic factors and treatment means of chronic liver diseases.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a method for establishing a rhesus monkey liver fibrosis model by intraperitoneal injection. Background technique [0002] Chronic liver disease is a major disease that endangers the health of the population. Hundreds of millions of people in my country carry hepatitis virus. On this basis, factors such as environmental pollution, grain mildew, general-purpose food additives, and alcoholic and non-alcoholic overeating are superimposed on each other, leading to inflammatory reactions, fatty liver, liver fibrosis, Among them, millions of people develop liver cirrhosis and liver cancer every year, causing huge social and family burdens. At present, traditional clinical drug treatment cannot improve the prognosis of end-stage liver disease, and the severe shortage of donor organs worldwide and the increasingly prominent contradiction between supply and d...

Claims

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Application Information

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IPC IPC(8): A61K31/02A61K47/44A01K67/02A23K50/00A23K20/158A23K20/147A23K20/20A23K20/26A23K20/24A23K20/10
CPCA01K67/02A23K20/10A23K20/147A23K20/158A23K20/20A23K20/24A23K20/26A23K50/00A61K9/0019A61K31/02A61K47/44
Inventor 黄孝伦冯天航赖春友魏玲玲施静袁可张恬莹
Owner SICHUAN PROVINCIAL PEOPLES HOSPITAL
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