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Sample pad, sample pad treatment liquid and test strip for detecting HIV antibody in urine

A technology of sample pad treatment liquid and test strips, which is applied in the field of medicine, can solve the problems of poor discrimination between negative and positive samples, and the inability to realize color development, etc., to eliminate the influence of false positives, improve the chromatographic form of the membrane surface, and improve the sensitivity. Effect

Active Publication Date: 2018-02-23
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different test samples and items, there are big problems when using the existing sample pad treatment solution for testing HIV antibody test strips in urine, for example: sample pad treatment solution for HIV blood test strips The sample pad treatment solution of , HCV and HBV test strips cannot achieve color development for HIV negative and positive samples, while the sample pad treatment solution of HIV saliva test strips has close color development for negative and positive samples, so the discrimination between negative and positive samples very poor

Method used

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  • Sample pad, sample pad treatment liquid and test strip for detecting HIV antibody in urine
  • Sample pad, sample pad treatment liquid and test strip for detecting HIV antibody in urine
  • Sample pad, sample pad treatment liquid and test strip for detecting HIV antibody in urine

Examples

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Effect test

Embodiment 1

[0043] Example 1 is used to detect the sample pad treatment liquid of the test strip of HIV in urine

[0044] The sample pad treatment solution of this embodiment includes Tris-HCl buffer solution, HPMC, PAA, rabbit serum, S9 and 0.09M Na with a pH of 9 and 100 mM 2 CO 3 ;

[0045] Wherein, the addition amount of HPMC was 0.1 g, the addition amount of PAA was 0.1 g, the addition amount of rabbit serum was 0.1 g, and the addition amount of S9 was 0.1 g per 100 mL of Tris-HCl buffer solution.

[0046] The preparation method of the sample pad treatment solution is:

[0047] Prepare Tris-HCl with a pH of 9 and 100mM as the base solution, then add HPMC and PAA, rabbit serum, S9, and then add Na 2 CO 3 , to obtain the sample pad treatment solution.

Embodiment 2

[0048] Example 2 is used to detect the sample pad treatment liquid of the test strip of HIV in urine

[0049] The sample pad treatment solution of this embodiment includes Tris-HCl buffer solution, HPMC, PAA, rabbit serum, S9 and 0.5M Na with a pH of 10 and 10 mM 2 CO 3 ;

[0050] Wherein, with respect to every 100mL of Tris-HCl buffer solution, the addition amount of HPMC is 0.7g, the addition amount of PAA is 0.3g, the addition amount of rabbit serum is 1g, and the addition amount of S9 is 1g.

[0051] The preparation method of the sample pad treatment solution is:

[0052] Prepare Tris-HCl with a pH of 10 and 10mM as the base solution, then add HPMC and PAA, rabbit serum, S9, and then add Na 2 CO 3 , to obtain the sample pad treatment solution.

Embodiment 3

[0053] Example 3 is used to detect the sample pad treatment liquid of the test strip of HIV in urine

[0054] The sample pad treatment solution in this embodiment includes Tris-HCl buffer solution, HPMC, rabbit serum, S9 and 1M Na with a pH of 11 and 200 mM 2 CO 3 ;

[0055] Wherein, relative to every 100 mL of Tris-HCl buffer solution, the addition amount of HPMC was 5 g, the addition amount of rabbit serum was 1 g, and the addition amount of S9 was 0.5 g.

[0056] The preparation method of the sample pad treatment solution is:

[0057] Prepare Tris-HCl with a pH of 11 and 200mM as the base solution, then add HPMC, rabbit serum, S9, and then add 1M Na 2 CO 3 , to obtain the sample pad treatment solution.

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Abstract

The invention discloses a sample pad, a sample pad treatment liquid and a test strip for detecting a HIV antibody in urine. The sample pad treatment liquid comprises a buffer solution with pH of 8-11,an aqueous rheological assistant and a sealing agent. Relative to each 100mL of the buffer solution, 0.05 to 10g of the aqueous rheological assistant and 0.05 to 10g of the sealing agent are used. The sample pad treatment liquid of the test strip for detecting a HIV antibody in urine utilizes the buffer solution with the appropriate pH and the aqueous rheological assistant and the sealing agent with appropriate concentrations, eliminates the urine influence on immune gold stability and improves an immune gold dissolution release rate and a membrane surface tomographic form.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically, the invention relates to a sample pad for detecting HIV antibody in urine, a sample pad processing solution and a test strip. Background technique [0002] Human immunodeficiency virus (Human Immunodeficiency Virus, HIV) is a virus that infects the cells of the human immune system. It destroys the immune cells of the human body and causes the immune system to lose its resistance, resulting in various diseases. According to statistics from the National Center for Disease Control and Prevention, since 2007, AIDS has become the infectious disease that has caused the highest death toll in China and continues to this day. It is worth noting that 32.1% of the infected people in China are still undetected. The only way to determine whether a person has HIV is to get an HIV test. Data show that the number of HIV tests in China increased rapidly between 2008 and 2015. According ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/531
CPCG01N33/531G01N33/56983G01N2333/155
Inventor 张秋平陈立李高辉
Owner GUANGZHOU WONDFO BIOTECH
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