Anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human colon cancer resisting drugs

A technology of Aspergillus versicolor and human colon cancer cells, applied in the field of biomedicine, can solve problems such as drugs that have not yet been found, and achieve the effect of inhibiting the proliferation activity of human colon cancer and significantly improving the proliferation activity of human colon cancer

Active Publication Date: 2018-02-27
FUJIAN HEALTH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that the anthraquinone compounds shown have anti-human colon cancer activity, and there is no repor...

Method used

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  • Anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human colon cancer resisting drugs
  • Anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human colon cancer resisting drugs
  • Anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human colon cancer resisting drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Fermentative production and separation and purification of the compound of embodiment 1

[0020] 1 Fermentation production

[0021] Fermentation culture of production bacteria: According to the conventional method of cultivating microorganisms, take Aspergillus versicolor ( Aspergillus versicolor ) IBPT-7 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2013715) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 2 to 3 days.

[0022] Take an appropriate amount of Aspergillus versicolor cultivated on a slant surface for 2 to 3 days, inoculate it into the SWS culture medium, and after 30 days of static culture at 28°C, obtain 20L of mycelium and fermentation broth; the composition of the culture fluid: 10.0 g of starch per liter of water, peptone 1.0 g, NaCl 20.0 g.

[0023] 2 Obtaining the extract

[0024] The Aspergillus versicolo...

Embodiment 2

[0030] Example 2 Test of Anti-tumor Activity in Vitro

[0031] 1 Experimental samples and experimental methods

[0032] Preparation of test sample solution The test sample is the pure product of the compound isolated and refined in the above-mentioned Example 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0033] The subculture of cell lines and cells adopts tumor cell lines, and the tumor cells are treated with DMEM medium containing 10% FBS at 37°C with 5% CO 2 Subculture in an incubator.

[0034] Cell Proliferation Inhibitory Activity Test Method

[0035] Tetrazolium salt (MTT) method Take tumor cells in the logarithmic growth phase and adjust the cell density to 1×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 μL per well, and placed in 5% CO at 37°C. 2Incubate for 4 h in an incubator. Add 2 μL of sample solution or blank solution to each w...

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Abstract

The invention relates to an anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human colon cancer resisting drugs. The compound plays a role in inhibiting proliferation of colon cancer cells. The compound has a structural formula shown in the description. The compound is obtained through carrying out fermentation culturing on aspergillus versicolorIBPT-7 so as to obtain a fermented product and then separating the compound from the fermented product and purifying the compound. Confirmed by experiments, the compound has relatively good anti-tumor activity to human colon cancer cells, i.e., HCT-116. The compound can be applied to research on human colon cancers as a human lung cancer cell proliferation inhibiting drug or anti-tumor drug.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to an anthraquinone compound derived from Aspergillus versicolor and its application in the preparation of anti-human colon cancer drugs. Background technique [0002] Anthraquinones are widely used in natural medicines and have important pharmacological effects. For example, Chinese medicine Polygonatum, Comfrey, Salvia, Rhubarb, Tripterygium and other plants contain a large amount of anthraquinones, which have anti-tumor, antibacterial, and antioxidant properties. It has multiple functions, especially in the prevention and treatment of major diseases such as cancer, cardiovascular disease, senile dementia, and AIDS. Among them, in the field of anti-tumor, its mechanism of action is mainly manifested in inducing tumor cell apoptosis, reversing the multidrug resistance of tumor cells, enzyme inhibitors, affecting the proliferation and cell cycle of tumor cells, inhibiting the m...

Claims

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Application Information

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IPC IPC(8): C07D313/20A61P35/00C12P17/08C12R1/66
CPCC07D313/20C12P17/08
Inventor 倪敏张丽华林燕喃唐小峦陈筱瑜
Owner FUJIAN HEALTH COLLEGE
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