Joint focusing method of microscope lens with different magnifications based on image texture

Abstract

The invention discloses a method for joint focusing of microscope lenses with different magnifications based on image texture, comprising the following steps: using a low magnification objective lens; initializing the Z-axis position; moving the stage downward for N steps, and calculating the image definition of the camera at equal intervals , record the maximum resolution and its Z-axis position; move the stage up to 90% of the distance between the current position and the position of the maximum resolution; determine the movement step of the stage according to the image resolution at this time, and repeat this step until The sharpness reaches a certain error range of the maximum sharpness; the effective area on the slide is divided into m×n blocks, the sharpness value of each sub-block is calculated, and the center of the sub-block with the largest sharpness value is moved to directly below the objective lens; The objective lens is changed to a high-magnification objective lens and a higher-magnification objective lens, and the above focusing process is repeated to finally complete joint focusing. The invention effectively avoids the problem of falling into a local extremum in the existing focusing method, and also solves the dilemma of not being able to focus because there is no target in the field of view.

Description

technical field [0001] The invention relates to the technical field of optical focusing, in particular to an image texture-based joint focusing method for lenses with different magnifications of a microscope. Background technique [0002] Based on the development of digital image processing and digital imaging systems, the development of fully automatic microscopes has gradually moved towards serialization, integration and intelligence, and is playing an increasingly important role in various fields such as life science, material science, and national defense security. Manual microscope inspection requires the operator to repeatedly adjust the focus plane. The whole process is time-consuming and labor-intensive. The focus definition depends on personal subjective evaluation and easily leads to errors. Therefore, automatic focus of fully automatic digital microscopes is imperative. [0003] The vigorous development of image processing technology and the maturation of automati...

AI Technical Summary

Problems solved by technology

However, in practical applications, the focus evaluation function curve has a local maximum value. When the hill-climbing search method encounters a local maximum value, it is easy to mistakenly think that the focal plane has been found.

In addition, most of the existing automatic focusing methods are to find the focal plane in a fixed field of view. If there are no or few focusing targets in the current field of view, the focus will fail.

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