Application of ethanol extract of bean taro leaves in reducing lipid deposition in liver cells

An ethanol extraction and lipid deposition technology, applied in the field of medicine, can solve the problems of easy rebound, reduce body immunity, etc., and achieve the effect of reducing the lipid deposition of HepG2 cells and having a good development prospect.

Active Publication Date: 2021-01-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the other weight loss and fat reduction methods also have the disadvantages of lowering the body's immunity and easy rebound.

Method used

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  • Application of ethanol extract of bean taro leaves in reducing lipid deposition in liver cells
  • Application of ethanol extract of bean taro leaves in reducing lipid deposition in liver cells
  • Application of ethanol extract of bean taro leaves in reducing lipid deposition in liver cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, a kind of preparation method of bean taro leaf ethanol extract, carries out following steps successively:

[0059] 1), mix taro leaves with 85% ethanol containing 0.1mol / L hydrochloric acid in the ratio of 1g:8ml, and use a juice extractor to beat; the obtained slurry is soaked by ultrasonic (40,000Hz ultrasonic frequency) at 45°C After extracting for 240 min, the extract was centrifuged at 4000 rpm for 30 min,

[0060] The filter residue obtained by centrifugation replaces the taro leaves in the above steps and repeats the beating, ultrasonic extraction and centrifugation of the above steps; the number of repetitions is 3 times;

[0061] The supernatants obtained by the above 3 times of centrifugation were combined to carry out the following step 2).

[0062] 2), the obtained supernatant was concentrated by rotary evaporation in a rotary evaporator (the process parameters set by the rotary evaporation were temperature 38°C, vacuum degree -0.09MPa) until ...

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Abstract

The invention discloses an application of ethanol extract of bean taro leaf in reducing lipid deposition in liver cells: it is used for preparing medicine and health food for treating or preventing non-alcoholic fatty liver. The preparation method of the ethanol extract of taro leaves comprises the following steps: mixing taro leaves with 85% ethanol containing 0.1mol / L hydrochloric acid, beating, ultrasonic extraction, and centrifugation to obtain a supernatant; The evaporator is used for rotary evaporation and concentration; the concentrate is separated and purified by macroporous resin, and methanol aqueous solution containing 0.05% formic acid is used as the eluent; the eluent is concentrated by rotary evaporation, and the obtained slurry concentrate is pre-frozen first, then vacuum Freeze drying machine to dry it into powder to obtain the ethanol extract of bean taro leaves.

Description

technical field [0001] The present invention relates to the field of medicine, in particular to the preparation of the ethanol extract of bean taro leaves and its application in the preparation of health food and medicine for the prevention and treatment of non-alcoholic fatty liver; at the same time, it relates to the reduction of oleic acid-induced lipid deposition in HepG2 cells in the prevention and treatment of Application in nonalcoholic fatty liver disease. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A23L33/105A61P1/16A61K127/00
CPCA23V2002/00A61K36/48A61K2236/333A61K2236/39A61K2236/51A61K2236/55A23L33/105A23V2200/32A23V2250/21
Inventor 郑晓冬楚强李永璐余露霜刘阳阳
Owner ZHEJIANG UNIV
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