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Porcine circovirus type 2 and porcine circovirus type 3 bivalent inactivated vaccine and preparation method thereof

A bivalent inactivated vaccine, porcine circovirus technology, applied in multivalent vaccines, biochemical equipment and methods, viruses, etc., to achieve the effect of long immunization cycle, less labor and cost reduction

Active Publication Date: 2018-10-12
陕西诺威利华生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no domestic or foreign registered PCV-3 vaccine and PCV-2 and PCV-3 bivalent vaccine

Method used

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  • Porcine circovirus type 2 and porcine circovirus type 3 bivalent inactivated vaccine and preparation method thereof
  • Porcine circovirus type 2 and porcine circovirus type 3 bivalent inactivated vaccine and preparation method thereof
  • Porcine circovirus type 2 and porcine circovirus type 3 bivalent inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the preparation of porcine circovirus type 2 and type 3 bivalent inactivated vaccine

[0027] 1. Origin of the strain

[0028] The selected porcine circovirus type 2 strain is PCV-2d strain HEB18, which has been preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation date: June 7, 2017, and the preservation number is CGMCC No. .13854.

[0029] The selected porcine circovirus type 3 strain is PCV-3 strain BJ23, which has been preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation date: June 7, 2017, and the preservation number is CGMCC No. .13855.

[0030] 2. Preparation and inspection of vaccine semi-finished products

[0031] 2.1 Preparation of seeds for production

[0032] 2.1.1 Preparation of porcine circovirus type 2: Dilute virus seeds appropriately with emulsion, inoculate PK15 cells (purchased from China Veterinary Drug ...

Embodiment 2

[0059] Example 2, the comparison of the immune protection effect of the bivalent inactivated vaccine product trial-produced in Example 1 and two kinds of monovalent inactivated vaccines and the serological effect evaluation

[0060] 1 Materials Example 1 Porcine Circovirus Type 2 and Type 3 Bivalent Inactivated Vaccine Trial Products; Porcine Circovirus Type 2 Inactivated Vaccine (SH Strain), produced by Pulaike Bioengineering Co., Ltd. (batch number 160616 ); porcine circovirus type 3 inactivated vaccine trial product.

[0061] 2 Animal experiment design

[0062] Select 70 weaned piglets at the age of 21 to 28 days, and divide them into 7 groups, 10 pigs in each group (see the table below); each pig in the 1st and 2nd groups is injected with the bivalent virus of porcine ring type 2 and type 3 respectively in the neck. 1ml of inactivated vaccine; 1ml of porcine circovirus type 2 inactivated vaccine was injected intramuscularly into the neck of each pig in the third group, an...

Embodiment 3

[0092] Research on the duration of immunity of trial-produced products in Example 3 and Example 1

[0093] 1 Materials Example 1 Porcine Circovirus Type 2 and Type 3 Bivalent Inactivated Vaccine Trial Products; Porcine Circovirus Type 2 Inactivated Vaccine (SH Strain), produced by Pulaike Bioengineering Co., Ltd. (batch number 160616 ); porcine circovirus type 3 inactivated vaccine trial product.

[0094] 2 Animal experiment design

[0095] Choose 70 weaned piglets of age of 21~28 days, be divided into 7 groups, every group 10, grouping treatment situation sees the following table:

[0096]

[0097] Note:

[0098] a. Vaccine 1 is the trial product of the bivalent inactivated vaccine of porcine circovirus type 2 and type 3 in Example 1; vaccine 2 is the inactivated vaccine of porcine circovirus type 2 (SH strain), Pulaike Bioengineering Co., Ltd. Produced by the company (batch number 160616); Vaccine 3 is a trial product of porcine circovirus type 3 inactivated vaccine. ...

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Abstract

The invention provides a porcine circovirus 2 and porcine circovirus 3 bivalent inactivated vaccine and a preparation method thereof. The bivalent inactivated vaccine comprises inactivated porcine circovirus 2 and 3 and a vaccine adjuvant, wherein the content of the porcine circovirus 2 and 3 is respectively at least 105.5 TCID50 / piece. The bivalent inactivated vaccine disclosed by the invention has the following advantages that the immune effect of the porcine circovirus 2 is equivalent to or better than that of a commercial vaccine, a porcine circovirus 3 commercial vaccine does not exist inthe market, and two antigens do not interfere with each other; the immune duration is long, and the efficacy is lasting; and only one shot immunization is needed, the cost is reduced, and stress reactions of animals are reduced. The bivalent inactivated vaccine disclosed by the invention can be applied to simultaneously preventing the porcine circovirus 2 and 3.

Description

technical field [0001] The invention relates to a porcine circovirus type 2 and type 3 bivalent inactivated vaccine and a preparation method thereof, belonging to the field of veterinary vaccines. Background technique [0002] Porcine circovirus (Porcine circovirus, PCV) is one of the smallest animal viruses discovered so far, with a diameter of 12-23nm. Based on the differences in nucleotide sequence, pathogenicity and antigenicity of porcine circovirus, it is divided into three subtypes, namely PCV-1, PCV-2 and PCV-3 genotypes, among which PCV-1 is It is considered non-pathogenic. The PCV2 genome size is divided into two types: 1767 and 1768bp. Currently, the PCV-2 genotype is mainly divided into five subtypes: 2a, 2b, 2c, 2d and 2e. The PCV3 genome size is 2000bp. PCV-3 can cause clinical infection in pigs of different ages. PCV-2 and PCV-3 are postweaning multisystemic wasting syndrome (Postweaning Multisystemic Wasting Syndrome, PMWS), PDNS (porcine dermatitis and nep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/116A61K39/12A61P31/20
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/55566A61K2039/70C12N2750/10034
Inventor 刘灿陈瑞张磊
Owner 陕西诺威利华生物科技有限公司
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