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Application of total flavonoid extract of tagetes patula in antiepileptic drug

A technology of anti-epileptic drugs and total flavonoids, which can be used in drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc., and can solve the problems of weakened curative effect and increased toxicity.

Inactive Publication Date: 2018-04-03
蔡德成 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the clinical treatment of epilepsy is mainly oral chemical synthetic drugs, and chemical drugs inevitably have side effects, most of which will have adverse effects on the nervous system, digestive system and blood system. Patients may experience dizziness, fatigue, nausea, A series of symptoms such as vomiting and thrombocytopenia, and the efficacy of long-term application is weakened and the toxicity is increased

Method used

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  • Application of total flavonoid extract of tagetes patula in antiepileptic drug
  • Application of total flavonoid extract of tagetes patula in antiepileptic drug
  • Application of total flavonoid extract of tagetes patula in antiepileptic drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: A kind of preparation method of total flavonoids of malachite grass with antiepileptic effect

[0019] Take the whole peacock herb, remove impurities, weigh 1kg, crush it, pass through a 20-mesh sieve, add 10L of 80% ethanol, extract twice in a water bath under reflux, each time for 2h, filter and combine the extracts, concentrate under reduced pressure to 1L, add petroleum ether (1L×3) extraction to obtain a degreasing concentrated solution. Add the degreased concentrated solution to the treated D101 macroporous adsorption resin, first elute with water for 5 column volumes, then with 60% ethanol for 5 column volumes, combine the ethanol eluents, concentrate and dry under reduced pressure to obtain The total flavonoids extract of Malachite herb is 116g, and the total flavonoids measured are 39.7g, accounting for 34.2% by weight of the total flavonoids extract after drying, and the percentage of the total weight of Malachite herb raw materials, that is, the...

Embodiment 2

[0020] Embodiment 2: A kind of preparation method of total flavonoids of malachite grass with antiepileptic effect

[0021] Take the whole peacock grass, remove impurities, weigh 1kg, crush it, pass through a 50-mesh sieve, add 12L of 70% ethanol, heat and reflux for 3 hours, extract twice in total, filter and combine the extracts, concentrate under reduced pressure to 1.5L, add petroleum Ether (1.5L×2) was extracted to obtain a degreased concentrated solution. The concentrated solution after degreasing was added to the treated HPD100 macroporous adsorption resin, first eluted with water for 4 column volumes, then with 80% ethanol for 5 column volumes, combined ethanol eluents, concentrated under reduced pressure and freeze-dried That is to say, 108g of the total flavonoids extract of Malachite, the total flavones recorded are 35.5g, accounting for 32.9% by weight of the total flavone extract after drying, and the percentage of the total weight of the raw materials of Malachit...

Embodiment 3

[0022] Embodiment 3: A kind of preparation method of total flavonoids of malachite grass with antiepileptic effect

[0023] Take the whole peacock herb, remove impurities, weigh 1kg, crush it, pass through a 100-mesh sieve, add 10L of 90% ethanol, reflux extraction for 2 hours, extract twice in total, filter and combine the extracts, concentrate under reduced pressure to 1L, add petroleum ether (1L×3) extraction to obtain a degreasing concentrated solution. The concentrated solution after degreasing was added to the treated AB-8 macroporous adsorption resin, and 5 column volumes were first eluted with water, followed by 6 column volumes with 80% ethanol. The ethanol eluents were combined and concentrated under reduced pressure. Spray drying to obtain 101g of the total flavonoids extract of Malavia flavonoids, the recorded total flavonoids is 31.5g, accounting for 31.2% by weight of the total flavonoids extract after the drying, and the percentage of the total weight of Malacoc...

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Abstract

The invention relates to an application of a total flavonoid extract of tagetes patula in antiepileptic drug. A method comprises the following steps: crushing whole herb of the tagetes patula, conducting reflux extraction by virtue of ethanol, and implementing degreasing by virtue of petroleum ether; and then, implementing macroporous adsorption resin purification, so that the total flavonoid of tagetes patula is obtained, wherein the content of the total flavonoid of tagetes patula is greater than 30%; and the total flavonoid of tagetes patula, together with medicinal adjuvants, can be prepared into granules, tablets or capsules as drugs for therapy or adjuvant therapy of epilepsy. Pharmacological tests prove that the total flavonoid is relatively good in pharmacological effect on a pentrazole-induced mouse acute epilepsy model, and the total flavonoid can obviously prolong an incubation period of pentrazole-induced mouse epileptic seizure, reduce the occurrence rate of tonic convulsion in experimental epileptic mice induced by pentrazole and prolong the death time of the pentrazole-induced epileptic mice.

Description

technical field [0001] The invention relates to a plant drug extraction method and application thereof, in particular to a drug extract with total flavonoids of malachite as active ingredients and its use in treating antiepileptic drugs. Background technique [0002] Peacock grass (Tagetespatula L.), also known as small marigold, red yellow grass, passionflower, stinky chrysanthemum, satin flower (Yunnan), is a plant of the genus Marigold in the Asteraceae family. It is an annual herb with a flowering period from July to September. Originally produced in Mexico, it has been naturalized in central and northwestern Yunnan, central and southwestern Sichuan, and western Guizhou. It is now cultivated in various places in my country. It is a common ornamental flower. Peacock grass has medicinal and health care functions, and is used as medicine in the Lisu and Yi nationalities. Its taste is bitter and flat, clearing away heat and promoting dampness, relieving cough. For cough, d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P25/08
CPCA61K36/28A61K2236/333A61K2236/35A61K2236/39A61K2236/51A61K2236/55
Inventor 蔡德成冉小库
Owner 蔡德成
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