Method for identifying resistance of tobacco to meloidogyne incognita

Abstract

The invention belongs to the technical field of plant genetic engineering and in particular relates to a method for identifying whether tobacco has resistance to meloidogyne incognita or not. The method comprises the following steps: preparing a PVY-NIB gene, and establishing a transient expression vector PVX:pND108-NIB; converting an agrobacterium strain, and preparing a transfection bacterial solution; performing dip dyeing on tobacco leaves, and identifying the resistance of the tobacco to the meloidogyne incognita. By virtue of the characteristic that a meloidogyne incognita resistant geneof tobacco is tightly interlinked with a PVY-MSNR gene, specific gene is utilized to convert tobacco, and the disease resistance of a variety to be detected is accurately and rapidly identified and judged according to phenotype differences of disease resistant and susceptible varieties. Compared with the prior art, the method has the active beneficial effects that the identification cycle is short, on-batch application is achieved, high identification efficiency is achieved, technical bases can be made for culture and screening of novel anti-disease varieties, and the like.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to a patent application for an identification method for identifying whether tobacco has resistance to root-knot nematode incognita. Background technique [0002] Tobacco root-knot nematode is one of the common diseases in tobacco production in all countries in the world. It has the widest occurrence range in my country, serious damage, and difficult control, which seriously restricts the development of my country's tobacco industry. Compared with other plant pathogens (fungi, bacteria, viruses), root-knot nematodes can actively invade and parasitize and transfer themselves, and at the same time, they can also interact with some pathogens to cause complex diseases. That is to say, root-knot nematodes have Characteristics of dual pathogens. Researchers investigated the occurrence of tobacco pests and diseases in the country in 2005, and the statistical r...

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Problems solved by technology

[0007] Aiming at the defects that the existing traditional resistance identification technology is difficult to adapt to the identification of a large number of breeding materials, and the identification results are greatly affected by the environment, the identification period is long, and the accuracy is not high, the purpose of this application is to provide a non-damaging, new Tobacco root-knot nematode resistance identification method, so as to realize simple, accurate, rapid, high-throughput screening and identification of resistance to tobacco root-knot nematode (Meloid-knot nematode incognita)

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