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Novel animal liver nucleic acid extraction kit and extraction method

A nucleic acid extraction reagent and animal liver technology, applied in the field of molecular biology, can solve the problems of high protein and fat content, poor purity of nucleic acid extraction solution, etc., and achieve a high degree of automation, no PCR inhibitory substances, and high nucleic acid recovery rate Effect

Inactive Publication Date: 2018-04-24
宁波卡尔新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, because the magnetic beads also have a certain degree of adsorption to protein and fat, the purity of the nucleic acid extraction solution is not good, and the protein and fat content is high.

Method used

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  • Novel animal liver nucleic acid extraction kit and extraction method
  • Novel animal liver nucleic acid extraction kit and extraction method
  • Novel animal liver nucleic acid extraction kit and extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of SG-TETA2.

[0039] [1] Synthesis of SG-TETA

[0040]Add 15ml CPTS (82mmol), 150ml methanol, and 25ml TETA (167mrn01) into a 250ml three-neck flask, stir magnetically, and reflux the mixture for 12h under nitrogen protection to obtain CPTS-TETA. Distill the mixture in the above-mentioned three-necked flask, then add 5 g of activated silica gel, use 150 ml of toluene as a solvent, mechanically stir, and reflux for 12 h under nitrogen protection. The product was washed successively with toluene and ethanol, and suction filtered. Put it in a Soxhlet extractor, extract with absolute ethanol under reflux for 24 hours, and dry it under vacuum at 50 degrees for at least 48 hours to obtain SG-TETA.

[0041] [2] Synthesis of SG-TETA-MA

[0042] Weigh 6.69 SG-TETA, place it in a 250ml three-neck flask, add 100ml methanol, 16ml (176mmol) redistilled MA, and react for 3d at 50°C under nitrogen protection. After the reaction, filter with suction, place in a Soxh...

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Abstract

The invention discloses a novel animal liver nucleic acid extraction kit and an extraction method which are characterized by fatty polyamine-type dendrimer (PAMAM) and ceramic particles. The kit comprises a lysis solution LB, a magnetic bead suspension, a washing solution and an eluent. The extraction kit is characterized by adding the fatty polyamine-type dendrimer and the ceramic particles simultaneously to effectively improve the removal of impurities such as proteins and fats and the like, and by means of the kit, extracted animal liver tissue genome nucleic acid has high recovery rate andpurity, complete fragments and no PCR inhibitors. The whole process of nucleic acid extraction from cracking, purifying to collecting of products can be completed by instruments, automation degree ishigh, a reagent used for extraction is high-efficient and safe, and damage to human body is avoided to the greatest extent.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a novel animal liver nucleic acid extraction kit and extraction method characterized by fatty polyamine dendrimers. Background technique [0002] Nucleic acid (Nucleic Acids) is the carrier of genetic information. People must extract and purify nucleic acid for gene recombination, transformation, evolution analysis, disease diagnosis and gene therapy. Therefore, the extraction, separation and purification of nucleic acid samples is an indispensable part of various experiments in molecular biology. The extraction method of nucleic acid has a great influence on the detection process and detection effect. [0003] Nucleic acid extraction is the basic method of molecular biology and the most critical method in nucleic acid diagnosis. It is the premise of downstream diagnosis, analysis and preparation. With the wide application of molecular detection technology, nucleic acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1013
Inventor 王科媛
Owner 宁波卡尔新材料科技有限公司
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