Mitochondrial genome complete sequence primer of octopus dollfusi as well as designing, phylogenetic analysis and amplification methods

A technology for mitochondrial genome and evolution analysis, applied in biochemical equipment and methods, biostatistics, computing, etc., to achieve the effect of reducing the number of primers and saving the cost of reagents

Inactive Publication Date: 2018-05-01
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the above-mentioned problem of not being able to amplify the full mitochondrial genome sequence of the octopus at present, a simple, fa

Method used

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  • Mitochondrial genome complete sequence primer of octopus dollfusi as well as designing, phylogenetic analysis and amplification methods
  • Mitochondrial genome complete sequence primer of octopus dollfusi as well as designing, phylogenetic analysis and amplification methods

Examples

Experimental program
Comparison scheme
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Embodiment

[0053] (1) Collection, identification and preservation of samples of octopus

[0054] The samples of the octopus used in the examples were all collected from the natural environment in the wild, and the collected specimens would be registered in the laboratory and identified with a stereo microscope to determine that they were octopuses. The species-identified octopus samples were soaked in 100% pure alcohol and stored in a refrigerator at 4°C for later use.

[0055] (2) Extraction of the mitochondrial genome of the bent octopus

[0056] The DNA drawer kit method was used to extract the mitochondrial genomic DNA of the octopus samples to be tested. In order to avoid the contamination of intestinal contents, the head and neck were discarded, and DNA was extracted only from the tentacles. DNA extraction was performed directly using a DNA extraction kit, and the extracted mitochondrial genomic DNA was stored at -20°C for future use.

[0057] (3) Design and synthesis of primers ...

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Abstract

The invention relates to a mitochondrial genome complete sequence primer of octopus dollfusi, which can achieve amplification of mitochondrial genome of the primer in one time and can rapidly acquirea genome complete sequence of the primer via sequencing and assembling and which has the characteristics of being simple, convenient and efficient, as well as designing, phylogenetic analysis and amplification methods; with the application of the primer provided by the invention, the mitochondrial genome of the primer can be amplified in one time and the genome complete sequence of the primer canbe rapidly acquired via sequencing and assembling, and the primer has the characteristics of being convenient and efficient; and the provided phylogenetic analysis of the mitochondrial genome completesequence primer of octopus dollfusi can offer important molecular means and data accumulation for population genetics, germ-plasm conservation and sustainable development and utilization of the octopus dollfusi as well as for phylogenic studies on octopus animals and even on entire cephalopoda.

Description

technical field [0001] The present invention relates to the field of molecular biology technology, in particular to a complete mitochondrial genome sequence of the octopus octopus that can be amplified at one time and can quickly obtain its complete genome sequence through sequencing and splicing, and has the characteristics of simplicity, speed and efficiency. Primers and design, phylogenetic analysis and amplification methods. Background technique [0002] Curved octopus is a very important economic species. Its carcass is oval, with smooth body surface and some network-like striations. There is a light-colored thin curved spot between the eyes, and a light-colored thick-striped spot in the center of the carcass. Short wrist type, the length of the wrist is about 3 or 4 times the length of the carcass, the length of each wrist is similar, and there are 2 rows of suction cups on the wrist. The third wrist on the right side of the male is stalked, shorter than the correspo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6869C12N15/11G06F19/24
CPCC12Q1/6869C12Q1/6888G16B40/00C12Q2531/113C12Q2565/125
Inventor 龚理闫允君吕振明刘立芹陈永久
Owner ZHEJIANG OCEAN UNIV
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