Tree tea tissue culture method

Abstract

The invention provides a tree tea tissue culture method which comprises the following steps: 1, preparing the material and method, wherein the test material is the second to third axillary buds of a new germinated branch of a tea tree in spring and is collected from a test tea garden of a tea research institute in Chinese Academy of Agricultural Sciences, and the variety of the tea is Longjing 43;2, carefully cleaning and disinfecting the collected branch with 2-3 axillary buds, cutting the branch into stem each of which is provided with an axillary bud and has the length of 1cm, inoculatingthe stems into a culture medium for germinating, and transferring on the novel identical medium for 3-4 times after germination, thereby obtaining the multiple shoots serving as an experimental material. According to the method disclosed by the invention, based on an aim of improving the proliferation rate of the multiple shoots of the tea tree and the planting percent, a hormone combination for the tree tea tissue culture and rapid propagation medium is optimized, so that the proliferation rate in each cycle of 20 days can reach 2.3 times or more, and more than 20% of buds can grow into plantlets with the height of 5cm or more suitable for transplanting.

Description

technical field [0001] The invention relates to tea tree technology, in particular to a tea tree tissue culture method. Background technique [0002] The tissue culture research of tea tree has already started from the 1980s, and the explants usually adopt leaf cotyledons, stem segments, axillary buds and the like. However, because tea tree is a perennial woody plant, its growth rate is slow [5], so there are problems of low proliferation rate and seedling rate. [0003] The tissue culture of tea tree has been studied by scientists since the 1880s. In recent years, Indra Sandal[7], Mondal[8] and others abroad have studied the tissue culture system of tea tree. hardly. But until now, its proliferation rate is low, growth rate is slow, and the problem of long culture cycle is still the bottleneck that tissue culture technology is used for tea tree productive seedling raising. Contents of the invention [0004] The object of the present invention is to, for the problems re...

AI Technical Summary

Problems solved by technology

But until now, the problems of low proliferation rate, slow growth rate and long culture cycle are still the bottlenecks of tissue culture technology for productive seedling cultivation of tea trees.