Liver fibrosis model as well as construction method and application thereof
A technology of liver fibrosis and construction methods, applied in the field of biological modeling, to achieve the effect of expanding application methods, promoting tissue damage repair/regeneration, and expanding the scope of application
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[0079] The present invention will be described in detail below in conjunction with examples. In order to make the objectives, technical solutions and advantages of the present invention clearer and clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments, but the present invention is not limited to these embodiments.
[0080] Unless otherwise specified, the raw materials and catalysts in the examples of the present invention are all purchased through commercial channels. Among them, Lgr5 (leucine-rich repeat-containing g-protein coupled receptor5) belongs to the G protein-coupled receptor family, and contains 17 leucine-rich repeats and 7 a-helix transmembrane regions. Its N- and C- The flanks of the terminal tail are rich in cysteine sequences, which can be expressed in various tissues such as the gastrointestinal tract and hair follicles.
[0081] The in-depth study of embryonic stem cells (ESC) and th...
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