Liver fibrosis model as well as construction method and application thereof

Abstract

The application discloses a liver fibrosis model as well as a construction method and an application thereof. The liver fibrosis model is induced by CCL4 through specific steps as follows: 8-week-oldmale mice are intraperitoneally injected with CCL4 in an amount of 3 ml/kg body weight, CCL4 is dissolved in olive oil in a ratio of 1:4, and control mice are only injected with olive oil twice a weekand injected continuously for 6 weeks; liver samples are taken 40 days after the first injection. Compared with mouse livers treated with oil, induction of target sites in the mouse livers treated with CCL4 is about 2-3 times, and the target expression reaches peak on the 5th day.

Description

Technical field [0001] The invention relates to the technical field of biological modeling, in particular to a liver fibrosis model and its construction method and application. Background technique [0002] The liver is a very important vital organ of the digestive system. It is responsible for regulating many important physiological activities, including glycogen storage, lipid metabolism, plasma protein secretion, foreign matter detoxification, bile secretion, etc. [0003] Liver functions are so diverse and important that once liver function is lost, it can cause organ failure, secondary hypotension, diabetes, brain damage, and even death within a few days. Liver diseases, for example, liver metabolic diseases, fulminant liver failure, hepatitis, cirrhosis, liver cancer, etc., are an important cause of death worldwide. [0004] At present, the current status of the treatment of liver diseases is not optimistic. Liver dialysis technology can cope with the threat of liver function ...

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Problems solved by technology

[0010] The use of stem cells to treat chronic liver fibrosis has not been reported so far

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