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Liver fibrosis model as well as construction method and application thereof

A technology of liver fibrosis and construction methods, applied in the field of biological modeling, to achieve the effect of expanding application methods, promoting tissue damage repair/regeneration, and expanding the scope of application

Inactive Publication Date: 2018-05-04
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The use of stem cells to treat chronic liver fibrosis has not been reported so far

Method used

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  • Liver fibrosis model as well as construction method and application thereof
  • Liver fibrosis model as well as construction method and application thereof
  • Liver fibrosis model as well as construction method and application thereof

Examples

Experimental program
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Embodiment Construction

[0079] The present invention will be described in detail below in conjunction with examples. In order to make the objectives, technical solutions and advantages of the present invention clearer and clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments, but the present invention is not limited to these embodiments.

[0080] Unless otherwise specified, the raw materials and catalysts in the examples of the present invention are all purchased through commercial channels. Among them, Lgr5 (leucine-rich repeat-containing g-protein coupled receptor5) belongs to the G protein-coupled receptor family, and contains 17 leucine-rich repeats and 7 a-helix transmembrane regions. Its N- and C- The flanks of the terminal tail are rich in cysteine ​​sequences, which can be expressed in various tissues such as the gastrointestinal tract and hair follicles.

[0081] The in-depth study of embryonic stem cells (ESC) and th...

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PUM

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Abstract

The application discloses a liver fibrosis model as well as a construction method and an application thereof. The liver fibrosis model is induced by CCL4 through specific steps as follows: 8-week-oldmale mice are intraperitoneally injected with CCL4 in an amount of 3 ml / kg body weight, CCL4 is dissolved in olive oil in a ratio of 1:4, and control mice are only injected with olive oil twice a weekand injected continuously for 6 weeks; liver samples are taken 40 days after the first injection. Compared with mouse livers treated with oil, induction of target sites in the mouse livers treated with CCL4 is about 2-3 times, and the target expression reaches peak on the 5th day.

Description

Technical field [0001] The invention relates to the technical field of biological modeling, in particular to a liver fibrosis model and its construction method and application. Background technique [0002] The liver is a very important vital organ of the digestive system. It is responsible for regulating many important physiological activities, including glycogen storage, lipid metabolism, plasma protein secretion, foreign matter detoxification, bile secretion, etc. [0003] Liver functions are so diverse and important that once liver function is lost, it can cause organ failure, secondary hypotension, diabetes, brain damage, and even death within a few days. Liver diseases, for example, liver metabolic diseases, fulminant liver failure, hepatitis, cirrhosis, liver cancer, etc., are an important cause of death worldwide. [0004] At present, the current status of the treatment of liver diseases is not optimistic. Liver dialysis technology can cope with the threat of liver function ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C07K19/00
CPCC12N5/067C12N2500/05C12N2501/12C12N2501/998C12N2506/14
Inventor 周伟杰林媛
Owner SOUTHERN MEDICAL UNIVERSITY
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