Caladium bicolor somatic embryogenesis path tissue culture rapid propagation method

Abstract

The present invention discloses a caladium bicolor somatic embryogenesis path tissue culture rapid propagation method, which comprises four steps such as selection and treatment of explants, inductionculture, proliferation and rooting culture, and transplanting, wherein the regenerated plant is obtained by using a somatic embryo path. According to the method, cleaned explants are inoculated on aninduction medium to culture callus, and the embryoid is differentiated from the callus so as to obtain a complete plant; and on the basis, the intact tissue culture seedlings as a proliferation testmaterial are inoculated on a culture mediums containing different concentrations of plant growth regulators, the optimal proliferation culture medium is screened by comparing under the same culture condition, and proliferation is performed while rooting is completed. According to the present invention, the selectivity of the explants is high, the treatment method is simple, and the sterile seedlings is obtained from the somatic embryo through the one-step process; and by improving the culture medium, the test steps and the method, the proliferation coefficient is obviously improved, the growthof the plant is promoted, and the survival rate of the transplanting was increased, such that the tissue culture propagation efficiency of caladium bicolor is improved.

Description

technical field [0001] The invention relates to a propagation method, in particular to a method for tissue culture and rapid propagation of the somatic embryogenesis pathway of Coleus variegata, belonging to the technical field of plant tissue culture propagation. Background technique [0002] Caiye taro, also known as colorful taro, belongs to the family Araceae and belongs to the genus Colorful taro. It is native to the tropical regions of South America and is most widely distributed in Brazil and the Amazon River Basin. Perennial herb with huge leaves, bright colors and high ornamental value. Because of its ever-changing leaf shape, leaf color and various markings, it is gorgeous and extraordinary, and it can grow well in indoor weak light, so it is a newly popular indoor foliage plant. [0003] At present, Coleus calyx mainly relies on tuber ramets for reproduction, but tuber reproduction is limited. A good tuber can produce at most 3-4 seedlings once a year, and the re...

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Problems solved by technology

[0003] At present, Coleus calyx mainly relies on tuber ramets for reproduction, but tuber reproduction is limited. A good tuber can produce up to 3-4 seedlings once a year, and the reproduction rate is low and the growth is slow.

Commercial reproduction can also be through seeds, but seed reproduction is difficult because the seeds are very small, the mortality rate is high, and the cost of plants obtained through seed growth and reproduction is too high

In addition, it has been reported that the offspring propagated by seeds are prone to variation and degeneration, and it is difficult to maintain the original traits

[0004] Since the use of conventional propagation methods cannot quickly and efficiently meet the needs of scientific research and large-scale production, it is necessary to study a rapid propagation method for Coleus cola

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