Rural household domestic sewage treatment device and sewage treatment method
A domestic sewage and treatment device technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, sustainable biological treatment, etc., can solve problems such as high cost, inconvenient use and maintenance, and poor treatment effect, and achieve Low cost, cost saving, and the effect of reducing clogging
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[0048] Set forth below the embodiment of the present invention, comprise the steps:
[0049] a. Sewage flows into the treatment device: the sewage from the septic tank 1 or the sewer pipe filters solid waste and then flows into the biological contact oxidation tank. According to the natural sewage production situation, natural or continuous water intake is acceptable. The intelligent controller 24 controls the water inlet pipe to pour The flow regulating valve 4 on the 2, when there is a lot of sewage, the excess sewage is temporarily stored in the septic tank 1 or a specially set buffer pool, and the influent flow is controlled at 200L-500L / d.
[0050] b. Bio-contact oxidation tank 5 hangs film to remove most of COD and part of ammonia nitrogen: elastic combined packing is set in biological contact oxidation tank 5, and continuous aeration is used for aeration: air flow meter detects the gas in the pipeline in real time Flow rate, the intelligent controller 24 compares the ga...
Embodiment 1
[0063] Configure each medium
[0064] Enrichment medium: 3g of beef extract, 10g of peptone, 5g of NaCl, 20g of agar, pH=7.2-7.4, 1L of water, sterilized at 121°C for 20min.
[0065] Bacterial medium: 5g of yeast extract, 10g of peptone, 0.5g of NaCl, 20g of agar, 1L of tap water, pH=7.0, sterilized at 121°C for 20min.
[0066] YPD medium: peptone 5g, glucose 10g, malt extract 3g, yeast extract 3g, agar 20g, water 1L, add 30μg / mL streptomycin or 100mg / L chloramphenicol, sterilize at 112°C for 30min;
[0067] Gaoshi No. 1 medium: 20g soluble starch, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl0.5g, FeSO 4 ·7H 2 O 0.01g, agar 20g, pH=7.4-7.6, sterilized at 121°C for 20min.
[0068] PDA medium: 200g potato, 20g glucose, 20g agar, 1L tap water, natural pH.
[0069] Primary screening medium: glucose 10g, K 2 HPO 4 5g, KH 2 PO 4 2g, NaCl 0.1g, MgSO 4 ·7H 2O 0.2g, urea 0.5g, yeast extract 0.5g, pH=7.2-7.5, water 1L, sterilized at 115°C for 20min.
[0070] Re...
Embodiment 2
[0072] Enrichment and Purification
[0073] Enrichment: Weigh 10g of the soil sample and add it to the sterile saline solution containing glass beads, shake well for half an hour, fully break up the soil sample, let it rest for clarification, absorb the supernatant and add it to the 90mL enrichment culture according to 10%. Base and 10ml of water samples in the culture medium. The enrichment solution was incubated at 30° C. and a shaker at 160 rpm for 1 day. Then the enrichment solution was serially diluted, that is, the supernatant was fractionally diluted to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 . Under aseptic operation, use a pipette to absorb 0.1mL of the above sample suspension, and spread it on YPD medium, Gao's No. 1 medium and PDA medium with a coating rod, and spread the dilution evenly . The plates were cultured upside down, and cultured at 30°C for 24-48 hours.
[0074] Purification: The enriched strains with different colony morphology...
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