Method and device for removing stray light in microscopic imaging based on prior photoelectric characteristics
A photoelectric characteristic and microscopic imaging technology, which is applied in the direction of measuring devices, color TV parts, TV system parts, etc., can solve problems such as complex algorithms, low experimental efficiency, and poor image clarity, and improve experimental efficiency. , reduce time complexity, improve the effect of image clarity
Active Publication Date: 2019-11-22
TSINGHUA UNIV
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Problems solved by technology
However, the experimental efficiency of related technologies is low, the picture definition is poor, and the algorithm is relatively complicated, which needs to be solved
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[0030] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.
[0031] The method and device for removing stray light based on photoelectric characteristic prior microscopic imaging according to the embodiments of the present invention will be described below with reference to the accompanying drawings. Stray light removal methods for imaging.
[0032] figure 1 It is a flow chart of a method for removing stray light in microscopic imaging based on prior photoelectric characteristics according to an embodiment of the present invention.
[0033] Such as figure 1 As shown, the stray light rem...
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The invention discloses a stray light removal method and device for microscopic imaging based on prior photoelectric characteristics, wherein the method includes: arranging a darkroom and taking points with logarithmic intervals on the exposure time parameter interval supported by the camera, and taking the first photo ;Only turn on the light source of the instrument in the darkroom, use light traps under the objective lens to absorb light, use the combination of camera exposure time and light source brightness, uniformly take points on the light source brightness, and take the same point at the camera exposure time, and take the second photo; Subtract the first photo from the second photo to eliminate the interference of the camera's own image sensor on the experimental results, and then obtain the change law of stray light in the exposure time of the camera and the brightness of the light source; analyze the intensity of stray light in a specific picture. When optimizing the distribution model in space, the stray light value of each pixel in the collected photos is obtained according to the change law, so as to remove the influence of stray light. This method can reduce the time complexity of the algorithm, and effectively improve the experimental efficiency and picture clarity.
Description
technical field [0001] The invention relates to the fields of computer vision technology and image processing technology, in particular to a stray light removal method and device for microscopic imaging based on prior photoelectric characteristics. Background technique [0002] The multi-dimensional, multi-scale and high-resolution computational imaging instrument is currently the world's largest computational imaging instrument that takes into account "wide field of view, high resolution, and high frame rate". Images can realize multi-dimensional, multi-scale and high-resolution continuous observation. The instrument requires that the photos taken by the instrument have a lower signal-to-noise ratio while meeting the frame rate requirements, and can display more details, in order to clearly observe the life movement at the cell level, thereby helping experimenters to grasp the life process The effective information in it not only saves operation time (the biggest difficult...
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IPC IPC(8): G01N21/01G06T5/00H04N5/21H04N5/232
CPCH04N5/21G06T5/001G01N21/01G06T2207/10061G06T2207/20032H04N23/80
Inventor 范静涛陈熙戴琼海
Owner TSINGHUA UNIV