A method for rapid propagation of virus-free potato seedlings in an open factory

Abstract

The invention discloses an open type factory rapid propagation method for detoxification seedlings of potatoes. According to the method, no culture medium is needed, so that a process of high-pressuresterilization of the culture medium is eliminated; no bacteriostatic agent is needed, so that the influence of the bacteriostatic agent on growth of the detoxification seedlings is avoided; detoxifying tissue-culture seedlings are directly propagated under an open and bacteria-containing environment without a super-clean worktable; the rapidly propagated stem cutting speed per capita is 10 timesor above that of a conventional mode; the propagation coefficient is 3 to 4 times; the propagation time is shortened to 14 to 15 days; the detoxification seedlings of the potatoes, which are culturedin an open and bacteria-containing greenhouse, can grow robustly; after the detoxification seedlings enter a breeder's seed production stage, the survival rate can be up to 100 percent; the factory rapid propagation production cost is 1 / 6 to 1 / 7 of that of the conventional mode; furthermore, the tuberization number is obviously higher than that of conventional detoxification seedlings. The open type factory rapid propagation method for the detoxification seedlings of the potatoes provides an important technical support for effectively reducing the production cost of factory rapid propagation of the detoxification seedlings of the potatoes, improving the propagation efficiency, producing robust and high-quality detoxification seedlings, planting and producing breeder's seeds, and producingbreeder's seeds through aeroponic culture or soilless culture.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of virus-free potato seedlings, and in particular relates to an open factory rapid propagation method of virus-free potato seedlings. Background technique [0002] Potatoes originated in Peru, South America, and are widely cultivated all over the world. Potatoes were introduced into my country in the late Ming and early Qing Dynasties, and have been cultivated in my country for nearly 400 years. Potato is an important grain and vegetable crop in the world. It has short growth period, wide adaptability, high yield, wide application, rich nutrition, and is resistant to storage and transportation. However, due to the attack of the virus, the potato is generally degraded during the cultivation process, resulting in a sharp decline in the quality and yield of the potato. There are many viruses that harm potatoes. Potatoes are asexually propagated crops. Viruses accumulate in potato tubers a...

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Problems solved by technology

[0005] In view of the harsh environmental requirements of the tissue culture room in the current process of cutting and growing seedlings of virus-free potato seedlings, it needs to be carried out under strict aseptic conditions, the operation procedures are cumbersome, low efficiency, high cost of cultivation, low survival rate of transplanting, and the number of tubers produced by cutting seedlings. In order to solve these problems, the technology of rapid propagation of virus-free potato seedlings has been systematically studied, and a new method of industrial rapid propagation of virus-free potato seedlings has been invented, which makes the rapid propagation of virus-free potato seedlings out of the strict aseptic operating environment. It is operated in a natural and open environment with bacteria, and at the same time, it simplifies the operation procedure of rapid propagation, which increases the propagation speed by more than 10 times compared with the traditional method, and greatly improves the quality and survival rate of virus-free potato seedlings. The production cost is lower than that of the traditional method. 1 / 6~1 / 7 of

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