Application of compound preparation of ascorbic acid and metformin in preparing chemotherapeutic drug
A metformin and ascorbic acid technology, applied in the field of biomedicine, can solve the problems of weak tumor cell inhibition, damage to normal cells, poor selectivity, etc., to reduce the incidence of serious adverse reactions, reduce side effects, and reduce medical costs Effect
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Embodiment 1
[0026] Such as figure 1 , figure 2 , Table 1, and Table 2, the present embodiment 1 is the group without any treatment as a control group, metformin is 10mM under the condition, the cell survival rate of different dosage regimens. First, metformin was formulated into a stock solution of 1 mol / L. Before adding the drug, it was diluted to 10 mM with DMEM culture solution containing 5% fetal bovine serum as needed to ensure that the volume occupied by double distilled water did not exceed 0.5%. The concentrations of ascorbic acid in each system were 10mM and 20mM respectively.
[0027] In terms of cell treatment, the cells in the logarithmic growth phase were taken, and 5×10 3 Cells / well were planted in a 96-well plate, and the total volume of culture medium in each well was 100 μl. After the cells had grown for 24 hours, different drug treatment regimens were given respectively. The cells were cultured for 24 hours, and after 24 hours, the cell viability was detected by sulf...
Embodiment 2
[0034] Such as figure 2 , 3, 4, and 5, in the present embodiment 2, the group without any treatment is used as the control group, and 20 mM metformin is added in the culture system, and the cell treatment, detection method, grouping, and legend are the same as in Example 1. The survival rate of the two cell lines was detected by SRB method. Since VC has a high inhibitory rate on tumor cells at 20mM, and the cell survival rate of normal cell lines and tumor cell lines differs greatly under the concentration of ascorbic acid, and when the dose of VC is 10mM, the above trend still exists, but the tumor cells The survival rate of the line is still higher than 50%, so 20 mMVC is selected as the VC concentration used in this embodiment, which is the optimal VC concentration. figure 1 Among them, the inhibitory rate of metformin added alone at 20 mM was significantly higher than that in Example 1 for various cells. Although the survival rate of human embryonic liver cell L-02 is ...
Embodiment 3
[0039] Such as figure 1 As shown, the cell treatment method in Example 3 is the same as that in Examples 1 and 2. The group without any treatment is used as the control group, 40 mM metformin is added to the culture system, and the survival rate of the two cell lines is detected by the SRB method . Such as figure 1 Shown, the survival rate of each cell under the situation of using 40mM metformin alone, the survival rate of normal cells is higher than that of cancer cells; as figure 2 As shown, the effect of the compound preparation of metformin and ascorbic acid on the survival of normal cells and tumor cells, as shown in the figure, the survival rate of L-02 is still higher than that of several other tumor cell lines, which shows that the compound preparation has a relatively high strong inhibitory effect.
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