Preparation of total phenolic acids from monkey earrings and its application in antibacterial and anti-inflammatory drugs

A technology of monkey earrings and total phenolic acids, which is applied in the direction of antibacterial drugs, anti-inflammatory agents, drug combinations, etc., can solve problems such as cumbersome steps, and achieve the effects of simple preparation method, easy production technology, and low production cost

Active Publication Date: 2021-08-10
辽宁昱众合医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is also a method for extracting total phenolic acids from monkey earrings in the prior art, such as CN201310666211.4 (hereinafter referred to as document 1), in which acid water is used to elute the macroporous resin, and the pH value needs to be adjusted repeatedly, and the steps are relatively cumbersome

Method used

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  • Preparation of total phenolic acids from monkey earrings and its application in antibacterial and anti-inflammatory drugs
  • Preparation of total phenolic acids from monkey earrings and its application in antibacterial and anti-inflammatory drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0018] The preparation of embodiment 1 monkey earring total phenolic acid

[0019] Take 100 g of monkey earrings, add 1000 ml of 60% ethanol aqueous solution to reflux extraction twice, each time for 2 hours, combine the two filtrates, concentrate under reduced pressure at 60 ° C to 5-10 g / L. Put this solution on a D101 macroporous resin column with a loading flow rate of 4BV / h, first elute 7BV with water at a flow rate of 6BV / h, and then elute 8BV with 70% ethanol aqueous solution, collect this part of the eluate, and depressurize Recover ethanol to 0.8-1.5g / L.

[0020] Put the sample concentrated by the macroporous resin column on the polyamide column, the loading flow rate is 2BV / h, first elute 5BV with water, the flow rate is 3BV / h, then elute 6BV with 80% ethanol aqueous solution, collect the ethanol eluate , ethanol was recovered under reduced pressure to obtain 11.9 g of total phenolic acids, which were spray-dried to obtain dry powder. The total yield of total phenol...

Embodiment 2

[0021] The preparation of the total phenolic acid of embodiment 2 monkey earrings

[0022] Take 100 g of monkey earrings, add 1000 ml of 70% ethanol aqueous solution to reflux extraction twice, each time for 2 hours, combine the two filtrates, concentrate under reduced pressure at 60 ° C to 5-10 g / L. Put this solution on a D101 macroporous resin column with a loading flow rate of 2BV / h, first elute 5BV with water at a flow rate of 5BV / h, and then elute 6BV with 80% ethanol aqueous solution, collect this part of the eluate, and depressurize Recover ethanol to 0.8-1.5g / L.

[0023] Put the sample concentrated by the macroporous resin column on the polyamide column, the sample loading flow rate is 4BV / h, first wash 3BV with water, the flow rate is 6BV / h, then elute 8BV with 70% ethanol aqueous solution, collect the ethanol eluate, Ethanol was recovered under reduced pressure to obtain 9.6 g of total phenolic acids, which were spray-dried to obtain dry powder. The total yield of ...

Embodiment 3

[0024] The preparation of the total phenolic acid of embodiment 3 monkey earrings

[0025] Take 100 g of monkey earrings, add 1000 ml of 50% ethanol aqueous solution to reflux extraction for 3 times, each time for 2 hours, combine the three filtrates, concentrate under reduced pressure at 60 ° C to 5-10 g / L. Put this solution on a D101 macroporous resin column with a loading flow rate of 3BV / h, first elute 6BV with water at a flow rate of 3BV / h, then elute 8BV with 60% ethanol aqueous solution, collect the ethanol eluate, and recover under reduced pressure Ethanol, to 0.8-1.5g / L.

[0026] Put the sample concentrated by the macroporous resin column on the polyamide column, the loading flow rate is 3BV / h, first elute 4BV with water, the flow rate is 4BV / h, then elute 6BV with 70% ethanol aqueous solution, collect the ethanol eluate , ethanol was recovered under reduced pressure to obtain 10.4 g of total phenolic acids, which were spray-dried to obtain dry powder. The total yie...

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Abstract

The invention discloses a method for preparing total phenolic acids from plant monkey earrings, which comprises the following steps: (1) pulverizing the monkey earring raw materials and extracting them with 50%-80% ethanol; (2) making large pores after the extract is concentrated Resin column, after washing with water, elute with 60%-80% ethanol, collect the eluate; (3) after the eluate is concentrated, put it on a polyamide column, wash with water, elute with 60%-80% ethanol, recover ethanol, dry, That is, the total phenolic acid of monkey earrings was obtained. The monkey earring total phenolic acids provided by the invention have antibacterial and anti-inflammatory activities, have strong antibacterial activities against Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, and Candida albicans, and can inhibit related inflammatory reactions at the same time. The method of the invention has the advantages of simple technological process, high yield and low cost, and provides broader market prospects for the development and utilization of monkey earrings.

Description

technical field [0001] The invention relates to the technical field of plant medicine, in particular to a preparation method of total phenolic acid (extract) in plant monkey earrings and a new application in the preparation of antibacterial and anti-inflammatory drugs. Background technique [0002] Antibacterial drugs refer to drugs that can inhibit or kill bacteria and are used to prevent and treat bacterial infections. At present, there are many antibacterial drugs in the market, including β-lactams, quinolones, aminoglycosides and other types of antibiotics. Due to the disadvantages of the traditional anti-infective drug treatment strategy and the serious problem of drug abuse, Under the pressure of antibiotic selection, drug-resistant bacteria such as MRSA (methicillin-resistant Staphylococcus aureus), Acinetobacter baumannii, and Klebsiella pneumoniae appeared, which caused a sharp increase in the mortality rate of infection and constituted a serious threat to the healt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P29/00A61P31/04A61P31/10G01N21/31
CPCA61K36/48A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55G01N21/31
Inventor 宋少江姜雪峰翟培伟张艳
Owner 辽宁昱众合医药科技有限公司
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