Application of cordyceps strain ferments as feed additive in improvement of reproductive performance of boars
A feed additive and reproductive performance technology, applied in the field of livestock and poultry breeding, to achieve the effect of improving reproductive performance
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Embodiment 1
[0042] 1. Fermentation liquid
[0043] (1) Cordyceps gunis strain: Acremonium terricola MKL18 (can be purchased from Hefei Miikeluo Biological Engineering Co., Ltd.);
[0044] (2) Seed cultivation:
[0045] The medium for seed culture is: 600g white granulated sugar, 300g silkworm chrysalis powder, 150g peptone, 200g soybean powder, 100g yeast powder, 10g MgSO 4 , 10g KH 2 PO 4 , dissolved in 20L water, natural pH; sterilized by high pressure steam at 121°C for 30min.
[0046] The conditions of seed fermentation are as follows: temperature is 25°C, ventilation rate is 1.00L / (L·min), inoculum size is 5% (mass fraction), liquid volume in 30L airlift fermentation tank is 67%, and fermentation time is 24h.
[0047] (3) Expansion of training:
[0048] The culture medium for expanded cultivation is: 6000g white granulated sugar, 3000g silkworm chrysalis powder, 1500g peptone, 2000g soybean powder, 1000g yeast powder, 100g MgSO 4 , 100g KH 2 PO 4 , dissolved in 200L water, na...
Embodiment 2
[0062] In this embodiment, Acremonium terricola (Acremonium terricola) MKL18 is used as the fermentation object for fermentation to obtain fermentation broth, fermentation powder and solid culture; except that the medium used for liquid fermentation is different from that of Example 1, the remaining contents are the same as those in Example 1. Example 1 is the same.
[0063] The medium for seed culture is: 600g white granulated sugar, 550g yeast powder, 200g soybean powder, 10g MgSO 4 , 10gKH 2 PO 4 , dissolved in 20L water, natural pH; sterilized by high pressure steam at 121°C for 30min.
[0064] The culture medium for expansion is: 6000g white granulated sugar, 5500g yeast powder, 2000g soybean powder, 100g MgSO 4 , 100g KH 2 PO 4 , dissolved in 200L water, natural pH; sterilized by high pressure steam at 121℃ for 30min.
[0065] The components in the fermentation broth, fermentation powder and solid culture prepared in this example were determined, and the results ar...
Embodiment 3
[0069] In this embodiment, Acremonium terricola (Acremonium terricola) MKL18 is used as the fermentation object for fermentation to obtain fermentation broth, fermentation powder and solid culture; except that the medium used for liquid fermentation is different from that of Example 1, the remaining contents are the same as those in Example 1. Example 1 is the same.
[0070] The medium for seed culture is: 600g white granulated sugar, 400g yeast powder, 200g soybean powder, 150g peptone, 10gMgSO 4 , 10g KH 2 PO 4 , dissolved in 20L water, natural pH; sterilized by high pressure steam at 121°C for 30min.
[0071] The culture medium for expansion is: 6000g white granulated sugar, 4000g yeast powder, 2000g soybean powder, 1500g peptone, 100g MgSO 4 , 100g KH 2 PO 4 , dissolved in 200L water, natural pH; sterilized by high pressure steam at 121℃ for 30min.
[0072] The components in the fermentation broth, fermentation powder and solid culture prepared in this example were d...
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