Cordyceps militaris mutant strain for obtaining high-yield cordycepin and application of cordyceps militaris mutant strain

A technology of cordycepin and cordyceps militaris, applied in the field of agricultural microorganisms, can solve the problems of economic loss, industrial development of strain degradation, poor quality, etc.

Active Publication Date: 2018-06-29
北京市辐射中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, the scale of artificial cultivation of Cordyceps militaris has continued to expand, but the degradation of the strain has always been a difficult problem restricting the development of the industry. The main performance is that it does not grow or only grows a very small number of fruit bodies, and the quality is poor, which will cause great economic losses.

Method used

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  • Cordyceps militaris mutant strain for obtaining high-yield cordycepin and application of cordyceps militaris mutant strain

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Embodiment 1

[0026] Example 1 Heavy carbon ion beam implantation mutagenesis breeding of Cordyceps militaris mutants with high yield of cordycepin

[0027] 1. Strains

[0028] The starting strain: Cordyceps militaris (Cordyceps militaris) No. CM-2, preserved by the Microbiological Laboratory of Beijing Radiation Center.

[0029] 2. Media and reagents

[0030] (1) culture medium

[0031] PDA medium: PDA powder (purchased from BD Company) 39g, distilled water to 1000mL, pH natural, sterilized at 121°C for 20min.

[0032] Liquid medium, seed liquid medium: 30g sucrose, 30g peptone, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate, with natural pH, heated to dissolve, distilled water to 1000mL, sterilized at 115°C for 30min.

[0033] Cordyceps militaris solid medium: 60g rice, add 60mL nutrient solution, and sterilize at 115°C for 30min.

[0034] Nutrient solution: glucose 20g / L, tryptone 15g / L, yeast powder 5g / L, potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 1.0g / L, pH...

Embodiment 2

[0078] Embodiment 2 The solid culture of Cordyceps militaris mutant strain CGMCC No.14800

[0079] 1. Solid culture of mutant strains

[0080] The solid culture of the Cordyceps militaris strain uses liquid seeds, and the preparation method of the seed liquid is as described in Example 1 for liquid culture. Take the cultured 5mL seed solution and evenly sprinkle it on the surface of the sterilized and cooled solid medium. Cultivate at a constant temperature of 25°C and avoid light. The surface of the medium is covered with mycelia for 7 days. The mycelium and rice grains on the upper layer of the medium are broken up into small particles with sterile tweezers in an ultra-clean bench, and spread flat in the culture bottle. Then move into the artificial climate incubator at 20-23°C, set daytime for 8h, humidity 80%, temperature at 23°C, light intensity at 1200lux, night at night for 16h, humidity at 75%, and temperature at 22°C. Yellow becomes orange, and yellow gradually deep...

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Abstract

The invention relates to a cordyceps militaris mutant strain for obtaining high-yield cordycepin and application of the cordyceps militaris mutant strain. A heavy carbon ion beam injection mutagenesistechnology is utilized for breeding a cordyceps militaris mutant strain CGMCC No.14800 for obtaining high-yield cordycepin and fruiting bodies, and the cordyceps militaris mutant strain serves as a fermenting strain for performing liquid fermentation to prepare the cordycepin; the maximum yield of the cordycepin in the obtained fermentation liquid can reach 929.390mu g / mL which is 8.15 times theyield of the cordycepin in the fermentation liquid obtained in the original strain; the strain CGMCC No.14800 is subjected to solid culture, and the maximum yield of the fruiting bodies can reach 22.376g / bottle which is 1.72 times the yield of the fruiting bodies of the original strain; and the maximum yield of the cordycepin in the fruiting bodies can reach 11777.094mu g / g which is 1.75 times theyield of the cordycepin in the fruiting bodies of the original strain. The invention provides a new way for development of cordyceps militaris strains for obtaining high-yield cordycepin.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a mutant strain of Cordyceps militaris with high yield of cordycepin and its application. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, is a model species of the genus Cordyceps. hotspot. [0003] Cordycepin is the most important bioactive component of Cordyceps militaris, which has antitumor, immune regulation, antibacterial, antiviral, and anti-inflammatory effects. Currently, the price of cordycepin on the market is expensive, mainly due to the limitation of conditions that can only be chemically synthesized in the laboratory, and large-scale production cannot be realized. The existing cordycepin on the market is mainly extracted and isolated from the culture of Cordyceps militaris. Therefore, the breeding of Cordycepin militaris strains with high yield plays an important role in re...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01H15/00C12P19/40A01G18/00C12R1/645
CPCA01H15/00C12N1/14C12P19/40C12N1/145C12R2001/645
Inventor 乔宇琛刘桂君周思静杨素玲王平宋梅芳孟佑婷顾海科武利勤郑洁
Owner 北京市辐射中心
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