Cordyceps militaris mutant strain for obtaining high-yield cordycepin and application of cordyceps militaris mutant strain

Abstract

The invention relates to a cordyceps militaris mutant strain for obtaining high-yield cordycepin and application of the cordyceps militaris mutant strain. A heavy carbon ion beam injection mutagenesistechnology is utilized for breeding a cordyceps militaris mutant strain CGMCC No.14800 for obtaining high-yield cordycepin and fruiting bodies, and the cordyceps militaris mutant strain serves as a fermenting strain for performing liquid fermentation to prepare the cordycepin; the maximum yield of the cordycepin in the obtained fermentation liquid can reach 929.390mu g / mL which is 8.15 times theyield of the cordycepin in the fermentation liquid obtained in the original strain; the strain CGMCC No.14800 is subjected to solid culture, and the maximum yield of the fruiting bodies can reach 22.376g / bottle which is 1.72 times the yield of the fruiting bodies of the original strain; and the maximum yield of the cordycepin in the fruiting bodies can reach 11777.094mu g / g which is 1.75 times theyield of the cordycepin in the fruiting bodies of the original strain. The invention provides a new way for development of cordyceps militaris strains for obtaining high-yield cordycepin.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a mutant strain of Cordyceps militaris with high yield of cordycepin and its application. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, is a model species of the genus Cordyceps. hotspot. [0003] Cordycepin is the most important bioactive component of Cordyceps militaris, which has antitumor, immune regulation, antibacterial, antiviral, and anti-inflammatory effects. Currently, the price of cordycepin on the market is expensive, mainly due to the limitation of conditions that can only be chemically synthesized in the laboratory, and large-scale production cannot be realized. The existing cordycepin on the market is mainly extracted and isolated from the culture of Cordyceps militaris. Therefore, the breeding of Cordycepin militaris strains with high yield plays an important role in re...

AI Technical Summary

Problems solved by technology

[0004] In recent years, the scale of artificial cultivation of Cordyceps militaris has continued to expand, but the degradation of the strain has always been a difficult problem restricting the development of the industry. The main performance is that it does not grow or only grows a very small number of fruit bodies, and the quality is poor, which will cause great economic losses.

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