Pharmaceutical composition of cannabidiol and vigabatrin and use thereof
A technology of cannabidiol and vigabatrin, applied in the treatment of West syndrome, in the field of compositions of cannabidiol and vigabatrin, can solve problems such as drowsiness, visual field defect, and blurred vision, and achieve relief Effects of side effects and good application prospects
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Embodiment 1
[0040] Example 1 Intraperitoneal injection of NMDA to establish an animal model of West syndrome
[0041] 1. Experimental animals
[0042] 12-day-old Wistar suckling mice, male or female, weighing (20±5) g, were fed by female mice under normal conditions, and all mice were kept on a standard 12 / 12-hour day / night cycle, with free access to food and water.
[0043] 2. Modeling Drugs
[0044] NMDA (N-methyl-D-aspartate, N-methyl-D-Aspartate).
[0045] 3. Modeling method
[0046] Take 12-day-old Wistar suckling mice, and inject NMDA (N-methyl-D-aspartate, N-methyl-D-Aspartate) 15 mg / kg intraperitoneally every day at 8:30-10:00 in the morning for 14 consecutive days . The animals were continuously observed for 2 hours after each injection, and corresponding records were made on the incubation period, seizure type, incidence rate and behavior changes of the epileptic seizures. The modeling was considered successful if the following manifestations occurred: Automatisms such as t...
Embodiment 2
[0047] Example 2 Effect of cannabidiol and vigabatrin in synergistic treatment of West syndrome
[0048] 1. Experimental method
[0049] On the day of the experiment, 40 Wistar suckling mice treated according to the method of Example 1 were randomly divided into four groups, administered within 30 minutes after the seizure, and then observed the performance of the tested animals after the administration.
[0050] Control group: 0.9% normal saline;
[0051] Combination drug group 1: Cannabidiol (200mg / kg) and vigabatrin (100mg / kg) composition;
[0052] Combination group 2: Cannabidiol (200mg / kg) and vigabatrin (10mg / kg) composition;
[0053] Combination group 3: Cannabidiol (20mg / kg) and vigabatrin (100mg / kg) composition;
[0054] Combination group 4: Cannabidiol (50mg / kg) and vigabatrin (100mg / kg) composition;
[0055] Cannabidiol group 1: (200mg / kg);
[0056] Cannabidiol group 2: (20mg / kg);
[0057] Cannabidiol group 3: (50mg / kg);
[0058] Vigabatrin group 1: (100mg / kg...
Embodiment 3
[0065] Example 3 Cannabidiol combined with vigabatrin reduces diarrhea symptoms
[0066] Establish the West syndrome suckling mouse model according to the method of embodiment 1 and divide into 3 groups at random according to body weight, every group 10, concrete grouping method is:
[0067] The control group was given an equal volume of normal saline;
[0068] Combination drug group 1: Cannabidiol (200mg / kg) and vigabatrin (100mg / kg) composition;
[0069] Combination group 2: Cannabidiol (200mg / kg) and vigabatrin (10mg / kg) composition;
[0070] Combination group 3: Cannabidiol (20mg / kg) and vigabatrin (100mg / kg) composition;
[0071] Combination group 4: Cannabidiol (50mg / kg) and vigabatrin (100mg / kg) composition;
[0072] Cannabidiol group 1: (200mg / kg);
[0073] Cannabidiol group 2: (20mg / kg);
[0074] Cannabidiol group 3: (50mg / kg);
[0075] Vigabatrin group 1: (100mg / kg);
[0076] Vigabatrin group 2: (10mg / kg).
[0077]The above-mentioned groups were administered ...
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