The present application relates to the field of analytical test technology, and particularly provides a
reagent and method for separating and analyzing C=C
double bond position isomerism based on
crown ether, which comprises: separating and analyzing 2 / 3-butenoic acid (BA), 2 / 3 / 4-pentenoic acid (PA) and 2 / 3 / 5-hexenoic acid (HA) isomers respectively, mixing the above unsaturated isomers with crown ethers including 18 crown 6 (18C6), 15 crown 5 (15C5), 12 crown 4 (12C4) and
divalent metal ions to form a
mixed solution, and then separating and analyzing the
mixed solution by means of
ion mobility
mass spectrometry. The
ion mobility
mass spectrometry experiment shows that the molecules can form ternary complexes of
crown ether-isomer-
metal ion through non-
covalent interaction, and the C=C position isomerism can be simultaneously separated through the formed complexes. The analytical
reagent and method provided by the present application are simple, accurate and fast in analysis speed, and do not need chemical
derivatization,
chromatographic separation and other previous steps, and the method can be used for separating different C=C
double bond position isomers.