A kind of preparation method of microbial agent carrier
A microbial inoculum and carrier technology, applied in biochemical equipment and methods, immobilized on/in organic carriers, chemical instruments and methods, etc., can solve difficult engineering applications, slow bacterial growth rate, environmental adaptability It can achieve high deodorization effect, improve biological activity, and improve microbial affinity.
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Embodiment 1
[0038] A preparation method of a microbial inoculum carrier, comprising,
[0039] S1, the corncob was added to the deionized aqueous solution containing sodium chlorite and glacial acetic acid, and the reaction was carried out at 80 °C for 3 h. After the reaction was completed, it was washed with distilled water until neutral, and dried to obtain delignified corncob; The consumption ratio of sodium chlorate, glacial acetic acid and deionized water is 1g:7mmol:3.8mmol:80mL;
[0040] S2, add deionized water to the delignified corncob, then add potassium permanganate for pre-oxidation at 60°C for 6 min, and then add concentrated sulfuric acid, N,N-methylenebisacrylamide and vigabatrin acid , reacted for 5h, washed 3 times with deionized water and absolute ethanol respectively, and dried to obtain modified corncob; wherein delignified corncob, deionized water, potassium permanganate, concentrated sulfuric acid, N,N-methylene The dosage ratio of bisacrylamide and vigabatrin is 1g:...
Embodiment 2
[0046] A preparation method of a microbial inoculum carrier, comprising,
[0047]S1, the corncob was added to the deionized aqueous solution containing sodium chlorite and glacial acetic acid, and the reaction was carried out at 65 °C for 5 h. After the reaction, rinsed with distilled water until neutral, and dried to obtain delignified corncob; The consumption ratio of sodium chlorate, glacial acetic acid and deionized water is 1g:6mmol:3.2mmol:60mL;
[0048] S2, add deionized water to the delignified corncob, then add potassium permanganate for pre-oxidation at 80°C for 8 min, and then add concentrated sulfuric acid, N,N-methylenebisacrylamide and vigabatrin acid , reacted for 2h, washed 3 times with deionized water and absolute ethanol respectively, and dried to obtain modified corncob; wherein delignified corncob, deionized water, potassium permanganate, concentrated sulfuric acid, N,N-methylene The dosage ratio of bisacrylamide and vigabatrin is 1g:100mL:0.4g:0.003mL:3mL...
Embodiment 3
[0052] A preparation method of a microbial inoculum carrier, comprising,
[0053] S1, the corncob was added to the deionized aqueous solution containing sodium chlorite and glacial acetic acid, and the reaction was carried out at 90 °C for 1.5 h. After the reaction, rinsed with distilled water until neutral, and dried to obtain delignified corncob; wherein the corncob, The consumption ratio of sodium chlorite, glacial acetic acid and deionized water is 1g:10mmol:5mmol:90mL;
[0054] S2, add deionized water to delignified corncob, then add potassium permanganate at 70°C for pre-oxidation for 10min, then add concentrated sulfuric acid, N,N-methylenebisacrylamide and vigabatrin acid , reacted for 6h, washed 5 times with deionized water and absolute ethanol respectively, and dried to obtain modified corncob; wherein delignified corncob, deionized water, potassium permanganate, concentrated sulfuric acid, N,N-methylene The dosage ratio of bisacrylamide and vigabatrin is 1g:85mL:0....
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