Anionic polypeptide carboxylated biological nano magnetic bead and preparation method thereof

An anionic polypeptide and bio-nano technology, applied in the field of nano-magnetic bead application and medical detection, can solve the problem that the performance of modified biological nano-magnetic beads varies greatly, the bacterial survival rate is low, the yield of nano-magnetic beads is low, and the success rate of DNA transformation and expression is low. and other problems, to achieve the effect of good anti-environmental impact ability, high practicability, and large amount of binding antibodies

Active Publication Date: 2018-07-06
BEIJING GUO KE RONG ZHI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the properties of the modified bio-nano-magnetic beads disclosed in the prior art after modification by nano-magnetic particles are quite different, the amount of bound antibodies is low, and the bio-nano-magnetic beads after grafting and modifying macromolecules have poor stability and are very easy to inactivate , resulting in difficulty in popularization and poor practicability
On the other hand, the genetic modification method is relatively easy to obtain biological nano magnetic beads similar to chemical synthesis, and the surface membrane can naturally have various functional groups, but in the preparation process of modified biological nano magnetic beads through genetic modification The conditions are very strict, and the success rate of DNA transformation expression is very low, the survival rate of strains and the yield of nano magnetic beads are both low, which inhibits its wide application

Method used

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  • Anionic polypeptide carboxylated biological nano magnetic bead and preparation method thereof
  • Anionic polypeptide carboxylated biological nano magnetic bead and preparation method thereof
  • Anionic polypeptide carboxylated biological nano magnetic bead and preparation method thereof

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Effect test

Embodiment 1

[0059] An anionic polypeptide carboxylated biological nano-magnetic bead, which is formed by fusion expression of an anionic polypeptide polymer grafted with polyethylene glycol and connected to the membrane protein of the biological nano-magnetic bead through a flexible linker. The amino acid residue of the flexible linker is GCVA (DLGGV) 2 GVC(GA) 3 MADEGAG; the amino acid residue of the anionic polypeptide polymer is

Embodiment 2

[0061] An anionic polypeptide carboxylated biological nano-magnetic bead, which is formed by fusion expression of an anionic polypeptide polymer grafted with polyethylene glycol and connected to the membrane protein of the biological nano-magnetic bead through a flexible linker. The amino acid residue of the flexible linker is GCVA (DLGGV) 2 GVC(GA) 3 MADEGAG; the amino acid residue of the anionic polypeptide polymer is

Embodiment 3

[0063] An anionic polypeptide carboxylated biological nano-magnetic bead, which is formed by fusion expression of an anionic polypeptide polymer grafted with polyethylene glycol and connected to the membrane protein of the biological nano-magnetic bead through a flexible linker. The amino acid residue of the flexible linker is GCVA (DLGGV) 2 GVC(GA) 3 MADEGAG; the amino acid residue of the anionic polypeptide polymer is

[0064] Preparation:

[0065] A. Construct a mutant strain of bacterial magnetic particle membrane protein gene mamC or mamF deletion, and obtain a first-class recombinant strain;

[0066] B. Anionic polypeptides are prepared by DNA synthesis, and the anionic polypeptides are fused with the bacterial magnetic particle membrane protein gene mamC or mamF through a flexible linker to form a gene fusion expression vector;

[0067] C. Import the expression vector obtained in step B into the primary recombinant strain obtained in step A, and screen out the se...

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Abstract

The invention provides an anionic polypeptide carboxylated biological nano magnetic bead, which is formed by connecting an anionic polypeptide polymer onto membrane protein of a biological nano magnetic bead through a flexible linker to carry out fusion expression; an amino acid residue of the flexible linker is GCVA(DLGGV)2GVC(GA)3MADEGAG; a specific anionic polypeptide structure is shown on thesurface of the biological nano magnetic bead; the quantity of provided functional groups serving as modification sites is large; the amount of bound antibodies is large, and the magnetic bead is highin bioactivity, higher in specificity and high in practicability.

Description

technical field [0001] The invention relates to the fields of nano magnetic beads application and medical detection, in particular to anionic polypeptide carboxylated biological nano magnetic beads and a preparation method thereof. Background technique [0002] Biological nanomagnetic beads are a kind of magnetic nanoparticles produced by magnetotactic bacteria, also known as bacterial magnetic particles, and the core is Fe 3 o 4 The crystal is coated with a layer of phospholipid biofilm outside, and the particle size is between 30-120nm. Biological nanomagnetic beads produced by the same magnetotactic bacteria have basically the same particle size and crystal form, uniform magnetic properties, natural biofilm coating, and good water-soluble and colloidal properties. In addition, the bacterial magnetic particles are bio-prepared, so they have good biocompatibility. There are a large number of functional groups on the surface membrane of biological nano magnetic beads, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12N15/62C12N15/63H01F1/00
CPCC12N15/1013C12N15/62C12N15/63H01F1/0045
Inventor 张金菊王红光
Owner BEIJING GUO KE RONG ZHI BIOTECH CO LTD
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