COX18 polypeptide with biological activity as well as synthesis method thereof and application thereof

A bioactive, solid-phase synthesis technology, applied in the field of biomedicine, achieves the effects of good compatibility, cost reduction, and simplified preparation steps

Inactive Publication Date: 2018-07-24
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the COX provided in the above two patents 52-69 There are still some areas that need further improvement in the performance of peptides

Method used

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  • COX18 polypeptide with biological activity as well as synthesis method thereof and application thereof
  • COX18 polypeptide with biological activity as well as synthesis method thereof and application thereof
  • COX18 polypeptide with biological activity as well as synthesis method thereof and application thereof

Examples

Experimental program
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Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0026] The reagents used in the solid-phase synthesis method of the biologically active COX18 polypeptide provided in this example are as follows:

[0027] 1. Wang Resin (0.47mmol / g) was purchased from Tianjin Nankai Hecheng Technology Co., Ltd.;

[0028] 2. Fmoc-Tyr(tBu)-OH and other amino acids protected by Fmoc were purchased from Shanghai Jier Biochemical;

[0029] 3. The condensation reagent N'-tetramethyluronium hexafluorophosphate (HBTU) was purchased from Shanghai Jier Biochemical (analytical pure);

[0030] 4.1-Hydroxy-phenylpropanoid-triazole (HOBt) was purchased from Shanghai Jier Biochemical (analytical pure);

[0031] 5. Diisopropylethylamine (DIEA) was purchased from Shanghai Jier Biochemical (analytical pure);

[0032] 6. Dimethylaminopyridine (DMAP) was purchased fr...

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Abstract

The invention provides a COX18 polypeptide with biological activity. An amino acid sequence of the polypeptide is as shown in SEQ ID NO. 1: LLPAGWILSH LETYRRPE. The invention further provides a methodfor synthesizing the COX18 polypeptide through a chemical synthesis method, namely an Fmoc method as well as application, and the polypeptide is applicationd for preparing drugs which inhibit sugar induced insulin secretion, resist insulin tolerance, resist hyperinsulinemia and reduce body weight. The invention finds a novel COX18 polypeptide which is 52-69th part of human cytochrome c oxidase 8A, which is different from the precious porcine sequence. Compared with the previous sequence, the sequence has 38.88% of different amino acid. Moreover, the sequence is humanized, and has better homology, fewer immunoreactions and side effects while being applied to a human body.

Description

technical field [0001] The present invention provides a new COX18 polypeptide and a process for synthesizing the COX18 polypeptide using the chemical synthesis method-Fmoc method. The COX18 polypeptide synthesized by this method is tested in vivo and in vitro in animals to prove that it has biological activity; and the present invention also provides The application of the COX18 polypeptide belongs to the technical field of biomedicine. Background technique [0002] short peptide COX 52-69 It is a polypeptide containing 18 amino acids. It was originally isolated and purified from the small intestine of pigs. After comparing the amino acid sequences, it was found that it completely overlaps with the 52nd to 69th parts of porcine cytochrome oxidase VIII, so it is named COX. 52-69 . Its sequence is LLPAGWVLSH LDSYKKRE. COX obtained by biochemical separation and purification 52-69 The functional experiment of the polypeptide found that the polypeptide can inhibit the secreti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02A61K38/44A61P5/50A61P3/04
CPCA61K38/44C12N9/0053C12Y109/03001
Inventor 李臣鸿雷维琼
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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