Preparation method and application of algae solution of euglena gracilis klebs for determining biotoxicity of particulates in water

Abstract

The invention relates to a preparation method and application of an algae solution of euglena gracilis klebs for determining the biotoxicity of particulates in water, and belongs to the field of environmental health toxicology. The preparation method comprises the following steps of culture of algae species and preparation of the algae solution, and specifically comprises the following steps: selecting the algae species of the euglena gracilis klebs, inoculating a container which contains a liquid culture medium with the algae species under a sterile condition, completing inoculating, culturing in a constant-temperature illumination incubator until a logarithmic phase, and standing for standby use; carrying out dark treatment on the algae solution which is cultured to the logarithmic phase, and determining OD680nm value by using a spectrophotometer or eliasa; drawing a cell growth curve according to the OD680nm value determined in step B, and selecting the algae solution of the euglenagracilis klebs of which the cell density range is 1*10<5> to 1*10<6> in the logarithmic phase per milliliter, thus determining the biotoxicity of the particulates in the water. The invention also provides determination of the algae solution of the euglena gracilis klebs in determining the biotoxicity of the particulates in the water. Through the preparation method provided by the invention, the biotoxicity of the particulates in the water can be directly expressed.

Description

technical field [0001] The invention belongs to the field of environmental health toxicology, and relates to the preparation of euglena fibril liquid, in particular to a preparation method and application of euglena fibril liquid for measuring the biological toxicity of water body particles. Background technique [0002] With the advancement of science and technology, the development of industrial and agricultural production, human beings have synthesized more and more chemicals, followed by the trend of various types and frequent occurrence of toxic pollutants entering the environment. The hazards tend to be complicated and comprehensive, and the resulting environmental problems are becoming more and more serious. Due to the rapid development of heavy polluting industries such as electroplating, tanning, pharmaceuticals, and chemicals, a large amount of industrial wastewater is discharged into urban sewage treatment plants, and the types of toxic substances in wastewater ar...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of inability to intuitively reflect the toxic performance of water particles on organisms in the prior art, the present invention provides a preparation method and application of euglena algae liquid for measuring the biological toxicity of water particles, which can fully understand the characteristics of water particles Toxic effects, and a deeper understanding of the toxic mechanism and pathways of particulate matter in water bodies, and better reflect the phenomenon and information of particulate matter in the actual water body environment

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