Establishment method of radix et rhizoma rhei quality evaluation system
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A technology for quality evaluation and establishment of methods, applied in the field of establishment of rhubarb quality evaluation system, can solve problems such as uneven quality of medicinal materials and obstacles to the effective use of rhubarb medicinal materials
Inactive Publication Date: 2018-08-10
CHONGQING MEDICAL & PHARMA COLLEGE
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Fengjie is one of the production areas of rhubarb, mainly home-grown horseshoe rhubarb, which is a kind of Sichuan rhubarb. The local rhubarb production is concentrated in October, and som...
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preparation example Construction
[0033] Preparation of reference substance solution: Accurately weigh the appropriate amount of emodin reference substance, rhein reference substance, and chrysophanol reference substance, and add methanol to make each 1ml containing 80ug of emodin, rhein, and chrysophanol respectively; precisely measure the above solutions respectively Put 2ml of each in a 10ml volumetric flask and mix well, and dilute to the mark with methanol to obtain the reference substance solution. Each 1ml of the reference substance solution contains 16ug each of emodin, rhein, and chrysophanol.
[0034]The first need testing solution prepared by the supercritical extraction method, the second need testing solution prepared by the solvent extraction method, and the prepared reference substance solution are injected into a high-performance liquid chromatograph using octadecylsilane bonded silica gel as a filler. The chromatographic column uses methanol-0.1% phosphoric acid solution as the mobile phase, th...
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[0050] Instruments and reagents
[0051] 1.1 Instrument: HA221-50-60 supercritical extraction device; Japan Shimadzu LC-15C high performance liquid chromatography.
[0052] 1.2 Reagents: Chrysophanol reference substance is provided by China Institute for the Control of Pharmaceutical and Biological Products, batch number: 110796-201621; emodin reference substance is provided by China Institute for the Control of Pharmaceutical and Biological Products, batch number: 110756-201610; Provided by the laboratory, batch number: 110757-201607.
[0053] Optimization of Extraction Process by Supercritical Extraction
[0054] Preparation of the test sample by supercritical extraction method: take the rhubarb medicinal material, pass through a 10-mesh sieve after crushing, take 120g, put it in an extraction kettle, add 190-240ml of 50%-95% ethanol as an entrainer, and put it under the condition of 41-49°C , maintain a pressure of 21-29MPa for 70-180 minutes. The extraction temperature ...
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Abstract
The invention provides an establishment method of a local radix et rhizoma rhei extraction technology and a quality evaluation system. The establishment method comprises the following steps: (1) preparing a test sample by a supercritical extraction method: taking a radix et rhizoma rhei medicinal material; after crushing, sieving and putting the material into an extraction kettle; feeding an entrainer and supercritical fluid, wherein the extraction temperature is 41 to 49 DEG C and the pressure is 21 to 29MPa; keeping for 70 to 180min to prepare radix et rhizoma rhei extract; (2) preparing a test sample solution, taking the radix et rhizoma rhei extract obtained by step (1) into a measuring flask and adding methanol to a scale; uniformly shaking and filtering; taking subsequent filtrate toprepare the test sample solution; (3) preparing a reference sample solution; (4) establishing a fingerprint spectrum through a high-performance liquid chromatography; (5) evaluating the similarity ofthe fingerprint spectrum. By adopting the establishment method provided by the invention, the extraction rate of a radix et rhizoma rhei effective component is easy to improve and various componentsare easy to extract; the quality evaluation system can be completed, the monitoring level is improved and the admission quality of radix et rhizoma rhei is improved.
Description
technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a method for establishing a rhubarb quality evaluation system. Background technique [0002] In order to develop and transform the traditional Chinese medicine industry, the country regards the Chinese medicine industry as a pillar industry for the development of the national economy, and invests huge sums of money to promote the modernization and industrialization of traditional Chinese medicine. However, the application and popularization of traditional Chinese medicine is restricted in many aspects. One of the reasons is the extraction of active ingredients of traditional Chinese medicine. The chemical composition of traditional Chinese medicine is very complicated and contains many kinds of active ingredients. Percolation method, reflux method, steam distillation method, etc., through extraction, separation and purification, effective monomers can be obtained. The...
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