The method for detecting oxidizing and reducing capacities of certain liquid in absence of
oxygen comprises the steps of mixing
agar and water, heating and dissolving the mixture to form an
agar solution in which
mass of the
agar accounts for 0.5% to 2% of the total
mass; adding a
potassium permanganate standard solution to the agar solution, moving the mixture in a transparent hard plastic box, and pressing and sealing the opening of the box by transparent soft plastics; placing the mixture at
room temperature, enabling the agar solution to form into a pink
potassium permanganate agar gel plate; adding the liquid to be detected dropwise by a
syringe above the center of the gel plate and through the transparent soft plastics, then enabling the liquid to be detected to diffuse immediately in the gel and be reacted with the
potassium permanganate, so that the
potassium permanganate is reduced and become colorless, and a colorless
diffusion ring is formed in the gel; measuring the
diameter of the
diffusion ring to acquire quantity and total reduction capacity of the object to be measured. The method for detecting oxidizing and reducing capacities of certain liquid in absence of
oxygen has the advantages of being capable of improving stability of experimental results remarkably, facilitating result reading, and controlling detection quality, suitable for micro-scale detections, simple and rapid in detection process, and applicable to intensification and commercialization integration methods.