Medicament for inhibiting proliferation of melanoma cells and application thereof

A technique for inhibiting melanin and tumor cells, applied in the fields of biomedicine and melanoma treatment, to achieve the effect of not reducing immunity, increasing apoptosis peak, and obvious effect

Pending Publication Date: 2018-08-17
TAISHAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, up to now, there is no literature report about 2,3-indoloquinone inhibiting the proliferation of melanoma cells, and there is no literature report about anti-melanoma cells when it is combined with other anticancer drugs.

Method used

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  • Medicament for inhibiting proliferation of melanoma cells and application thereof
  • Medicament for inhibiting proliferation of melanoma cells and application thereof
  • Medicament for inhibiting proliferation of melanoma cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0023] Embodiment 1-4 is in order to illustrate in vitro cell line experimental data:

[0024] The B16 cell lines were divided into four groups: control group, 2,3-indole quinone group, 5-fluorouracil group and combined drug group. Six wells in each group were treated for 48 hours. Use a flow cytometer with a fixed volume of 300ul / well, the same speed, and a fixed sampling time of 120s / well to detect cell number, cell cycle, apoptosis, and Bcl-2 expression.

[0025] Example 1 is the control group, with 1% DMSO added.

Embodiment 2

[0026] Example 2 is 2,3-indolequinone group, 2,3-indolequinone is prepared into 100mM mother solution with dimethyl sulfoxide (DMSO): the relative molecular mass of 2,3-indolequinone is 147, Therefore, weigh 14.7mg of 2,3-indolequinone and dissolve it in 1ml of DMSO, and the resulting solution is 100mM 2,3-indolequinone mother solution. Diluted to 200uM, 400uM, 800uM respectively with 1640 medium containing 10% fetal bovine serum.

Embodiment 3

[0027] Example 3 is the 5-fluorouracil group. Dissolve 5-fluorouracil with DMSO into a 100mM mother solution: weigh 13mg of 5-fluorouracil powder and dissolve it in 1ml DMSO, and the resulting solution is 100mM 5-fluorouracil mother solution. Dilute to 200uM with 1640 medium containing 10% fetal bovine serum for use.

[0028] Example 4 is a combination drug group, adding 400uM 2,3-indoloquinone and 200uM 5-fluorouracil (containing 1% DMSO).

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Abstract

The invention belongs to the field of biomedicine, and relates to a medicament for inhibiting the proliferation of melanoma cells. The active components include 2,3-indolequinone and / or 5-fluorouracil. The 2,3-indolequinone promotes B16 cell apoptosis and inhibits the growth of the melanoma cells by down-regulating the expression of bcl-2 proteins without reducing immunity or causing side effects.The 2,3-indolequinone can block the B16 cell cycle in the S cycle, namely, a DNA (Deoxyribonucleic Acid) synthesis cycle, and a sub-G1 apoptosis peak occurs. After the 2,3-indolequinone is used together with the 5-fluorouracil, the apoptosis peak increases remarkably, and the cycle arrest effect is more remarkable. The 2,3-indolequinone has no significant inhibitory effect on the number of immunecells in vivo, and shows a certain effect of relieving 5-fluorouracil immunosuppression after combination with the 5-fluorouracil. Thus, a new solution is provided for more effective treatment of themelanoma cells.

Description

technical field [0001] The invention belongs to the field of biomedicine, especially the field of melanoma treatment, and specifically relates to a medicine for inhibiting the proliferation of melanoma cells and an application thereof. Background technique [0002] Malignant melanoma (MM), referred to as malignant melanoma, is one of the most common malignant tumors worldwide. It originates from human neural crest melanocytes and is transformed from melanocytes. It is highly invasive, highly transfer characteristics. According to incomplete statistics, the incidence of human malignant melanoma accounts for 3% of the overall human malignant tumors, and the incidence of human skin malignant melanoma accounts for 7%-20% of all human skin malignant tumors, and its incidence is still around 100% per year. 4%-6% growth rate. [0003] So far, the treatment methods for human malignant melanoma mainly include surgery, radiation therapy, chemotherapy, biological therapy and tumor va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/404A61K31/513A61P35/00
CPCA61K31/404A61K31/513A61P35/00A61K2300/00
Inventor 张继国赵斌王晓丹唐华李雅琳
Owner TAISHAN MEDICAL UNIV
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