Method for creating red kidney bean mutant by using EMS (Ethy Methan Sulfonate) mutagenic agent and application thereof

Abstract

The invention belongs to the technical field of kidney bean breeding and particularly relates to a method for creating a red kidney bean mutant by using an EMS (Ethy Methan Sulfonate) mutagenic agentand the application thereof. The method comprises steps of seed pretreatment, mutagenesis treatment and seeding culture, wherein the step of mutagenesis treatment comprises steps of alternatively soaking sterile seeds into a plant growth regulator and a 0.01-0.05% EMS solution, wherein the soaking time of the plant growth regulator is 5-10minutes, the soaking time of the EMS solution is two timesof that of the plant growth regulator, and alternative soaking is carried out for 2-5 times; and sucking off moisture on the surfaces of the seeds so as to obtain primary mutagenesis seeds; radiatingthe primary mutagenesis seeds with rays so as to obtain secondary mutagenesis seeds; sowing and culturing the seeds, and screening the red kidney bean mutant. The method provided by the invention is aspecial efficient mutagenesis method, the mutagenesis efficiency can be improved, the amount of cancerogen such as EMS can be reduced, and the health threatening upon seed breeding operators can be reduced.

Description

technical field [0001] The invention belongs to the technical field of kidney bean breeding, and in particular relates to a method and application of using an EMS mutagen to create red kidney bean mutants. Background technique [0002] Red kidney bean is an angiosperm of the leguminous family. It has large grains and bright color. It has both nutritional and medicinal values, and is nutritious and healthy. Red kidney beans are rich in anthocyanins and saponins, which can reduce the content of local inflammatory tissues in joints, have obvious anti-inflammatory effects, and have anti-inflammatory and analgesic effects on arthritis patients. Red kidney beans are a specialty product in Shanxi, my country. The planting area of ​​red kidney beans in Kelan County reaches 130,000 mu, and the red kidney beans can be exported to earn 3.5 million US dollars, which greatly increases farmers' income. Maintaining the quality of the existing red kidney beans and developing new red kidney...

AI Technical Summary

Problems solved by technology

[0004] EMS is a commonly used plant mutagen. EMS belongs to a kind of alkylating agent and has high mutagenic efficiency. In the prior art, the usual volume fraction of EMS mutagen is 0.1-1.0%, but this substance has strong carcinogenicity and Volatile, when used, strict protection must be done, otherwise there will be a serious threat to the health of breeders

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